Related Experiment Video
Updated: Jun 30, 2025
11:11
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
Published on: February 17, 2016
11.9K
PRRC2B modulates oligodendrocyte progenitor cell development and myelination by stabilizing Sox2 mRNA
Ying Zhang1, Zhihong Song1, Rong Wu2
1Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Cell Reports
|March 20, 2024
Summary
PRRC2B regulates oligodendrocyte progenitor cell (OPC) development and myelination by stabilizing Sox2 mRNA. This finding offers a potential therapeutic target for myelin-related neurological disorders.
Area of Science:
- Neuroscience
- Molecular Biology
- Developmental Biology
Background:
- Oligodendrocyte progenitor cells (OPCs) are crucial for myelin production and neuronal function.
- Dysregulation of OPC development is linked to neurological diseases.
- The role of N6-methyladenosine (m6A) in OPC development is not fully understood.
Purpose of the Study:
- To investigate the mechanism by which m6A regulates OPC development.
- To identify the role of PRRC2B in OPC development and myelination.
Main Methods:
- Nestin-Cre-mediated Prrc2b deletion in mice.
- Oligodendroglia lineage-specific deletion of Prrc2b.
- Integrative analyses including methylated RNA immunoprecipitation sequencing, RNA sequencing, and RNA immunoprecipitation sequencing.
Main Results:
- PRRC2B acts as an m6A reader regulating OPC development and myelination.
- Deletion of Prrc2b impairs neural stem cell self-renewal and glial differentiation.
- PRRC2B stabilizes Sox2 mRNA, impacting OPC numbers, myelination, and motor function.
Conclusions:
- PRRC2B is a key regulator of OPC development and myelination through posttranscriptional control of Sox2.
- PRRC2B functions with PRRC2A to stabilize target mRNAs.
- PRRC2B represents a potential therapeutic target for myelin-related disorders.
Keywords:
CP: Developmental biologyCP: NeurosciencePRRC2Bm(6)A modificationmyelin-related disordersmyelinationoligodendrocyte progenitor cellMore Related Videos
09:32Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
Published on: May 21, 2018
13.7K
12:29Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
Published on: April 16, 2018
9.3K
Related Concept Videos
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
01:33Pleiotropy
40.4K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.4K
01:45Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
01:29Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
02:24Regulation of Expression Occurs at Multiple Steps
22.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.7K
01:23Regulation of Expression at Multiple Steps
904
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
904