Related Experiment Video

Updated: Jun 30, 2025

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization
08:13

Mitigation of Blood Borne Cell Attachment to Metal Implants through CD47-Derived Peptide Immobilization

Published on: December 3, 2020

4.6K

Expression of a mutant CD47 protects against phagocytosis without inducing cell death or inhibiting angiogenesis

Lu Xu1, Xiaodan Wang1, Ting Zhang1

  • 1Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, Institute of Immunology, First Hospital of Jilin University, Changchun, Jilin 130062, China; National-Local Joint Engineering Laboratory of Animal Models for Human Disease, Jilin University, Changchun, Jilin 130062, China.

Cell Reports. Medicine
|March 20, 2024
PubMed

Insights

Researchers developed a modified CD47 protein (CD47-IgV) to prevent transplant rejection without harmful side effects. This engineered protein protects cells from immune attack, offering promise for xenotransplantation and regenerative medicine.

Area of Science:

  • Immunology
  • Regenerative Medicine
  • Transplantation Biology

Background:

  • CD47 is a cell surface protein that interacts with SIRPα, an inhibitory receptor on immune cells.
  • Overexpressing CD47 is explored to prevent transplant rejection by inhibiting immune cells like macrophages.
  • The negative impacts of standard CD47 signaling are often disregarded in this therapeutic approach.

Purpose of the Study:

  • To engineer a modified CD47 protein (CD47-IgV) that retains protective functions without detrimental signaling.
  • To evaluate the efficacy and safety of CD47-IgV in preventing phagocytosis and its impact on cell functions.
  • To explore the potential of CD47-IgV for applications in xenotransplantation and regenerative medicine.

Main Methods:

  • Constructed a CD47 mutant (CD47-IgV) by fusing the CD47 IgV domain to a membrane anchor.
  • Expressed CD47-IgV on human and mouse cells.
  • Assessed CD47-IgV expression, phagocytosis inhibition in vitro and in vivo, cell death, angiogenesis, and hematopoietic stem cell engraftment and differentiation.

Main Results:

  • CD47-IgV was efficiently expressed on the cell surface of both human and mouse cells.
  • CD47-IgV protected cells from phagocytosis in vitro and in vivo.
  • CD47-IgV did not induce cell death, inhibit angiogenesis, or affect hematopoietic stem cell engraftment and differentiation.

Conclusions:

  • CD47-IgV represents a potentially safer method for achieving the benefits of CD47 overexpression.
  • This modified CD47 protein may facilitate the production of gene-edited pigs for xenotransplantation.
  • CD47-IgV holds promise for generating hypoimmunogenic pluripotent stem cells for regenerative medicine.
Keywords:
CD47angiogenesismacrophagephagocytosisstem celltransgenetransplant rejectiontumor

More Related Videos

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells08:46

A Real-time Potency Assay for Chimeric Antigen Receptor T Cells Targeting Solid and Hematological Cancer Cells

Published on: November 12, 2019

53.3K
Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo08:19

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

6.0K

Related Concept Videos

Phagocytosis of Apoptotic Cells01:17

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or  immature dendritic cells. Non-professional phagocytes such as  epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes. 
Normal cells contain receptors that prevent them from being recognized...
3.8K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K