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Related Experiment Video

Updated: Jun 30, 2025

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Published on: October 7, 2010

HIV-Tocky system to visualize proviral expression dynamics.

Omnia Reda^1,2, Kazuaki Monde³, Kenji Sugata¹

¹Division of Genomics and Transcriptomics, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan.

Communications Biology

|March 21, 2024

Summary

Understanding human immunodeficiency virus type 1 (HIV-1) latency is crucial. A new HIV reporter model, HIV-Tocky, distinguishes directly latent and recently silenced cells, offering insights into HIV reservoirs.

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Last Updated: Jun 30, 2025

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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

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Published on: October 16, 2018

Area of Science:

Virology
Immunology
Molecular Biology

Background:

HIV-1 latency establishment remains poorly understood.
The HIV reservoir consists of infected cells evading immune and viral clearance.
Existing in vitro models have limitations in analyzing provirus silencing dynamics.

Purpose of the Study:

To introduce a novel HIV reporter model, HIV-Timer of cell kinetics and activity (HIV-Tocky).
To enable the dissection of provirus silencing and reactivation dynamics.
To characterize distinct latent HIV populations.

Main Methods:

Development of the HIV-Tocky reporter model with dual fluorescence.
Utilizing spontaneous fluorescence shifting to track provirus silencing.
Analyzing integration features of latent cell populations.

Main Results:

HIV-Tocky can reveal provirus silencing and reactivation dynamics.
Identification of two distinct latent populations: directly latent and recently silenced.
The recently silenced subset exhibits integration characteristics indicative of stable latency.

Conclusions:

The HIV-Tocky model provides a novel tool for studying HIV latency.
It addresses the heterogeneous nature of HIV reservoirs.
Offers new avenues for evaluating HIV eradication strategies.