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Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of

Maryam Abtin1, Nahid Nafisi2, Asghar Hosseinzadeh1

  • 1Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Non-Coding RNA Research
|March 21, 2024
PubMed

Insights

Circular RNAs (circRNAs) and their role in breast cancer are explored. The circ_0009910/miR-145-5p/MUC1 axis impacts breast cancer progression and may offer new therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are novel regulatory RNAs with conserved sequences.
  • circRNAs play roles in cancer pathogenesis through complex regulatory axes.
  • The specific function of circ_0009910 in breast cancer remains to be fully elucidated.

Purpose of the Study:

  • To investigate the expression and functional role of the circ_0009910/miR-145-5p/MUC1 axis in breast cancer.
  • To determine the correlation between this axis and clinicopathological features of breast cancer.

Main Methods:

  • Quantitative real-time PCR was used to assess expression levels of circ_0009910, miR-145-5p, and MUC1 in breast cancer tissues and cell lines.
  • Clinicopathological data analysis was performed to correlate molecular findings with patient characteristics.
  • Functional assays, including siRNA-mediated knockdown of circ_0009910, were conducted to evaluate its impact on cell proliferation and migration.

Main Results:

  • circ_0009910 and MUC1 were upregulated, while miR-145-5p was downregulated in breast cancer tissues compared to controls.
  • Upregulation of circ_0009910 correlated with lymph node invasion.
  • Downregulation of miR-145-5p was associated with lymph node invasion and HER2-negative status. MUC1 overexpression correlated with younger patient age.

Conclusions:

  • The circ_0009910/miR-145-5p/MUC1 axis is implicated in breast cancer pathogenesis.
  • circ_0009910 knockdown reduced breast cancer cell proliferation and migration, suggesting its oncogenic role.
  • This axis represents a potential therapeutic target for breast cancer treatment.
Keywords:
Breast cancerCirc_0009910MUC1miR-145-5p

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