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Collagen modifications predictive of lymph node metastasis in dogs with carcinoma in mixed tumours

  • 0Laboratory of Comparative Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Frontiers in Veterinary Science +

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March 21, 2024

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March 21, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Canine mammary mixed tumours with lymph node metastasis show altered collagen fibre characteristics and increased COX-2 expression, indicating a role in tumour progression and poorer survival.

Area Of Science

  • Veterinary Pathology
  • Oncology
  • Biomaterials Science

Background

  • Canine mammary mixed tumours are common but some metastasize, impacting prognosis.
  • Understanding the factors driving metastasis is crucial for improving canine cancer treatment.

Purpose Of The Study

  • To investigate the role of collagen fibre characteristics and COX-2 expression in canine mammary tumours with and without metastasis.
  • To identify potential biomarkers for predicting metastatic potential in canine mammary mixed tumours.

Main Methods

  • Retrospective analysis of 46 canine mammary tumour samples (benign, carcinoma without metastasis, carcinoma with metastasis) and 23 normal mammary glands.
  • Multiphoton microscopy and advanced imaging techniques to analyze collagen fibre structure.
  • Immunohistochemistry for Ki67, ER, PR, HER-2, and COX-2 to assess cellular proliferation and immunophenotype.

Main Results

  • Metastatic canine mammary carcinomas (CMTM) exhibit shorter, wavier collagen fibres compared to non-metastatic carcinomas (CMT).
  • Carcinomas show reduced collagen fibre coverage and increased cellularity versus normal and benign tumours.
  • Strong COX-2 expression correlates with high cell proliferation and altered fibre characteristics in metastatic tumours.

Conclusions

  • Collagen fibre architecture is significantly altered in metastatic canine mammary carcinomas.
  • COX-2 expression is associated with tumour progression and metastatic potential in these neoplasms.
  • These findings underscore the importance of the tumour microenvironment, particularly collagen, in canine mammary cancer metastasis.

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