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A core genome multi-locus sequence typing scheme for Streptococcus uberis: an evolution in typing a genetically
Daniel Whiley1, Keith Jolley2, Adam Blanchard1
1School of Veterinary Medicine and Science, University of Nottingham, Nottingham, UK.
Microbial Genomics
|March 21, 2024
Summary
A new core genome multi-locus sequence typing (cgMLST) scheme for Streptococcus uberis, a cause of bovine mastitis, was developed. This scheme reveals the global genetic diversity of this important dairy cattle pathogen.
Area of Science:
- Veterinary Microbiology
- Genomics
- Epidemiology
Background:
- Streptococcus uberis is a significant cause of bovine mastitis, affecting dairy industry sustainability worldwide.
- Effective control strategies are hindered by a lack of detailed epidemiological data on S. uberis.
Purpose of the Study:
- To develop and implement a high-resolution core genome multi-locus sequence typing (cgMLST) scheme for Streptococcus uberis.
- To analyze the global genetic diversity and population structure of S. uberis.
Main Methods:
- Sequencing of 579 S. uberis isolates and incorporation of 305 public genomes from 11 countries.
- Development of a cgMLST scheme based on a core genome of 1475 genes and 1447 curated loci.
- Phylogenetic analysis of 932 distinct core genome sequence types (cgSTs) derived from 1012 isolates.
Main Results:
- The S. uberis cgMLST scheme successfully typed over 97% of isolates, yielding 932 unique cgSTs.
- Phylogenetic analysis showed limited geographical clustering, with a notable UK-centric clade but overall dispersed distribution.
- No clear clustering based on disease status or isolation year was observed, highlighting broad genetic diversity.
Conclusions:
- The developed cgMLST scheme provides a powerful new tool for detailed epidemiological analysis of S. uberis.
- The study confirms the significant global genetic diversity of Streptococcus uberis populations.
- Understanding this diversity is crucial for developing targeted interventions against bovine mastitis.
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