Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

12.9K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
12.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reamed and unreamed intramedullary nailing for the treatment of open and closed tibial fractures: a subgroup analysis of randomised trials.

International orthopaedics·2009
Same author

Selective COX-2 inhibitor versus nonselective COX-1 and COX-2 inhibitor in the prevention of heterotopic ossification after total hip arthroplasty: a meta-analysis of randomised trials.

International orthopaedics·2009
Same author

[Study on evaluating sex determining region of the Y as an engrafting track of BMSCs transplantation for repairing osteonecrosis of the femoral head of rabbit].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2009
Same author

Positive association between benign familial infantile convulsions and LGI4.

Brain & development·2009
Same author

Catalytic enantioselective synthesis of chiral phthalides by efficient reductive cyclization of 2-acylarylcarboxylates under aqueous transfer hydrogenation conditions.

Organic letters·2009
Same author

Significance of urinary liver-fatty acid-binding protein in cardiac catheterization in patients with coronary artery disease.

Internal medicine (Tokyo, Japan)·2009
Same journal

Corrigendum to "CFPNet-M: A light-weight encoder-decoder based network for multimodal biomedical image real-time segmentation" [Comput. Biol. Med. 154 (2023) 106579].

Computers in biology and medicine·2026
Same journal

ECG arrhythmia classification via wavelet-driven feature extraction and swarm-optimised gradient boosting.

Computers in biology and medicine·2026
Same journal

Electro-osmotic metachronal cilia transport of viscoelastic blood infused with penta-hybrid nanoparticles in an oviduct: Analytical and neural network modeling.

Computers in biology and medicine·2026
Same journal

sEEGnal: an automated EEG preprocessing pipeline evaluated against expert-driven preprocessing.

Computers in biology and medicine·2026
Same journal

Corrigendum to "Integrating experimental biology, computational methods, and artificial Intelligence in anticancer drug discovery: Bridging the translational Gap" [Comput. Biol. Med. 213 (2026) 111832].

Computers in biology and medicine·2026
Same journal

Organ dose optimization for a point-of-care forearm X-ray photon-counting CT.

Computers in biology and medicine·2026
See all related articles

Related Experiment Video

Updated: Jun 30, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K

Deciphering ACE2-RBD binding affinity through peptide scanning: A molecular dynamics simulation approach.

Jiahu Tang1, Ruibin Hu2, Yiyi Liu3

  • 1Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou, 511436, China.

Computers in Biology and Medicine
|March 21, 2024
PubMed
Summary
This summary is machine-generated.

This study identifies a key peptide fragment for SARS-CoV-2 binding to ACE2 using computational and experimental methods. This rapid peptide microarray approach aids in understanding virus entry and developing peptide therapeutics.

Keywords:
Molecular dynamics simulationPeptidesPlasmonic materialsProtein-protein interactionsSARS-CoV-2

More Related Videos

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.0K
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.4K

Related Experiment Videos

Last Updated: Jun 30, 2025

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.8K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.0K
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.4K

Area of Science:

  • Biochemistry
  • Immunology
  • Computational Biology

Background:

  • Protein-protein interactions (PPIs) are crucial in biological processes and disease.
  • Identifying binding epitopes for PPIs is vital for drug discovery and diagnostics.
  • Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) spike receptor-binding domain (RBD) interaction with human angiotensin-converting enzyme 2 (ACE2) is key to viral entry.

Purpose of the Study:

  • To investigate the epitope information of SARS-CoV-2 RBD binding to ACE2.
  • To develop a rapid and cost-effective method for identifying critical peptide fragments involved in PPIs.
  • To validate computational predictions with experimental data.

Main Methods:

  • Utilized theoretical calculations including docking and molecular dynamics simulations.
  • Employed peptide microarray technology based on plasmonic materials chip for experimental validation.
  • Compared the developed method with phage display technology and surface plasmon resonance (SPR).

Main Results:

  • Computational simulations identified peptide fragment #14 (YNYLYRLFRKSNLKP) from RBD as having the highest binding strength to ACE2.
  • Peptide microarray experiments confirmed the theoretical findings, validating fragment #14's strong binding.
  • The combined theoretical and experimental approach proved rapid and cost-effective.

Conclusions:

  • The study successfully identified a high-affinity peptide fragment of SARS-CoV-2 RBD for ACE2 binding.
  • Peptide microarray technology offers a fast and economical platform for epitope mapping in PPIs.
  • This methodology can accelerate the development of diagnostics, vaccines, and peptide-based therapeutics for infectious diseases.