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Updated: Jun 30, 2025

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
Published on: September 19, 2018
A domain swapping strategy to create modular transcriptional regulators for novel topology in genetic network
Clement T Y Chan1, Vincenzo Kennedy2, Sahaj Kinshuk2
1Department of Biomedical Engineering, University of North Texas, TX 76207, USA; BioDiscovery Institute, University of North Texas, TX 76207, USA.
Abstract:
Transcriptional regulators generate connections between biological signals and genetic outputs. They are used robustly for sensing input signals in building genetic circuits. However, each regulator can only generate a fixed connection, which generates constraints in linking multiple signals for more complex processes. Recent studies discovered that a domain swapping strategy can be applied to various regulator families to create modular regulators for new signal-output connections, significantly broadening possibilities in circuit design. Here we review the development of this emerging strategy, the use of resulting modular regulators for creating novel genetic response behaviors, and current limitations and solutions for further advancing the design of modular regulators.
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