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Related Concept Videos

Barrett Esophagus-I: Introduction01:21

Barrett Esophagus-I: Introduction

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Barrett's esophagus is a medical condition where the esophageal mucosa is significantly damaged by stomach acid or other digestive fluids, often due to long-term exposure associated with gastroesophageal reflux disease (GERD). In GERD, a weakened or abnormally relaxed lower esophageal sphincter allows stomach acid to flow persistently into the esophagus.
This constant acid exposure transforms the esophagus's pink mucosal lining (stratified squamous epithelium) into a type of lining more...
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Barrett Esophagus-II: Clinical Manifestations and Management01:21

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Individuals with Barrett's esophagus are often asymptomatic, but they may experience symptoms commonly associated with GERD, such as heartburn and acid regurgitation. Additional symptoms can include difficulty swallowing, chest pain, unintentional weight loss, blood in the stool (which may appear black, tarry, or bloody), and episodes of vomiting.
To diagnose Barrett's esophagus, healthcare providers often recommend an endoscopy for those showing symptoms of acid reflux. The procedure...
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Endoscopic Procedures I: Esophagogastroduodenoscopy01:29

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An Esophagogastroduodenoscopy (EGD) is a diagnostic procedure in which an endoscopist uses a flexible, lighted endoscope to visualize the upper gastrointestinal (GI) tract. The procedure includes visualizing the oropharynx, esophagus, stomach, and the first part of the small intestine, the duodenum.
During an EGD, the endoscope can be used to:
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Related Experiment Video

Updated: Jun 30, 2025

Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging
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Algorithm Training and Testing for a Nonendoscopic Barrett's Esophagus Detection Test in Prospective Multicenter

Prasad G Iyer1, Seth W Slettedahl2, Douglas W Mahoney2

  • 1Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota.

Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association
|March 21, 2024
PubMed
Summary
This summary is machine-generated.

A new 3-methylated DNA marker (MDM) panel algorithm using a non-endoscopic cell collection device (CCD) shows high accuracy for detecting Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC). This non-invasive test offers excellent sensitivity for high-risk BE cases.

Keywords:
Esophageal CancerPrognosisRiskScreening

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Area of Science:

  • Gastroenterology
  • Oncology
  • Molecular Diagnostics

Background:

  • Endoscopic detection of Barrett's esophagus (BE) and esophageal adenocarcinoma (EAC) is invasive and costly.
  • Non-endoscopic methods are guideline-recommended alternatives for BE/EAC detection.
  • A previously developed 5-methylated DNA marker (MDM) panel utilized cell collection device (CCD) specimens.

Purpose of the Study:

  • To train and validate a new algorithm using a 3-MDM panel for BE/EAC detection.
  • To assess the performance of the 3-MDM panel algorithm in an independent cohort.
  • To establish a less invasive diagnostic tool for BE and EAC.

Main Methods:

  • Algorithm training and testing were conducted on samples from two prospective, multicenter cohorts.
  • DNA was extracted from cell lysates collected via CCD, bisulfite treated, and MDMs were assayed.
  • A logistic regression model was trained and locked using cross-validation, then tested on an independent dataset.

Main Results:

  • The final 3-MDM panel (NDRG4, VAV3, ZNF682) demonstrated high sensitivity and specificity in both training and test sets.
  • Overall sensitivity for BE detection was 82%-88%, with 100% sensitivity for high-grade dysplasia and EAC.
  • Areas under the receiver operating characteristic curves (0.92-0.94) indicate strong diagnostic performance.

Conclusions:

  • A validated 3-MDM panel algorithm using non-endoscopic CCD shows excellent sensitivity for detecting high-risk BE.
  • This non-invasive approach provides a promising tool for BE/EAC screening and diagnosis.
  • The study provides strong evidence for the clinical utility of this novel diagnostic method.