Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Dual Nature of Electromagnetic (EM) Radiation01:10

Dual Nature of Electromagnetic (EM) Radiation

2.0K
Electromagnetic (EM) radiation consists of electric and magnetic field components oscillating in planes perpendicular to each other and mutually perpendicular to radiation propagation through space. EM radiation can be classified as a wave, characterized by the properties of waves such as wavelength (denoted as λ) and frequency (represented by ν).
Wavelength is the distance between two consecutive peaks (the highest point) or troughs (the lowest point) in the wave. Frequency is the...
2.0K
Interaction of EM Radiation with Matter: Spectroscopy01:12

Interaction of EM Radiation with Matter: Spectroscopy

1.5K
Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study...
1.5K
Mesh Analysis for AC Circuits01:12

Mesh Analysis for AC Circuits

374
In the domain of radio communication, the significance of impedance matching must be considered. It is crucial to ensure the efficient transmission of signals between radio transmitters and receivers. Achieving this balance involves using impedance-matching circuits, with one fundamental configuration comprising a resistor, capacitor, and inductor.
The process of harmonizing these impedances begins with a clear understanding of the input and output signals. Once these signals are known, the...
374
Electrostatic Boundary Conditions in Dielectrics01:27

Electrostatic Boundary Conditions in Dielectrics

1.2K
When an electric field passes from one homogeneous medium to another, crossing the boundary between the two mediums imparts a discontinuity in the electric field. This results in electrostatic boundary conditions that depend on the type of mediums the field propagates through.
Consider a case where both the mediums across a boundary are two different dielectric materials. Recall that the electric field and electric displacement are proportional and related through the material's...
1.2K
Electromagnetic Fields01:30

Electromagnetic Fields

2.1K
Electric fields generated by static charges, often referred to as electrostatic fields, are characteristically different from electric fields created by time-varying magnetic fields. While the former is a conservative field, implying that no net work is done on a test charge if it goes around in a complete loop in the field, the latter is, by definition, not a conservative field; net work is done, and it is proportional to the rate of change of magnetic flux.
However, the observation of...
2.1K
Back EMF01:24

Back EMF

2.9K
Generators convert mechanical energy into electrical energy, whereas motors convert electrical energy into mechanical energy. A motor works by sending a current through a loop of wire located in a magnetic field. As a result, the magnetic field exerts a torque on the loop. This rotates a shaft, extracting mechanical work from the electrical current sent in initially. When the coil of a motor is turned, magnetic flux changes through the coil, and an emf (consistent with Faraday's law) is...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic Perspectives on the Relationship Between Paraspinal Muscles and Bone Mineral Density: A Narrative Review.

Diagnostics (Basel, Switzerland)·2026
Same author

Molecular mechanism of IF1- and IF2-driven translation initiation in bacteria.

bioRxiv : the preprint server for biology·2026
Same author

Efficacy and safety of compound Qingdai enema in rapidly alleviating inflammatory activity in ulcerative colitis: a prospective study.

Frontiers in pharmacology·2026
Same author

Liquid Chromatographic Methods for Microsampling in Sports Drug Monitoring: Progress in DBS and VAMS.

Biomedical chromatography : BMC·2026
Same author

Titrated Contrast Recanalization: A Novel Controlled-Contrast Technique for Anterograde Crossing of Chronic Total Occlusion.

The American journal of cardiology·2026
Same author

Bilateral staghorn calculi in primary hyperoxaluria type 1.

Kidney international·2026

Related Experiment Video

Updated: Jun 30, 2025

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
08:47

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation

Published on: January 12, 2024

1.5K

Structural insights into human EMC and its interaction with VDAC.

Mingyue Li1,2, Chunli Zhang1,2, Yuntao Xu1,2

  • 1Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Aging
|March 22, 2024
PubMed
Summary

The endoplasmic reticulum membrane protein complex (EMC) structure reveals a gating plug that may regulate its diverse functions. This finding offers insights into how the EMC stabilizes and traffics membrane proteins.

Keywords:
EMCVDACcryo-electron microscopygating plugmembrane proteins biogenesis

More Related Videos

Finite Element Modelling of a Cellular Electric Microenvironment
08:23

Finite Element Modelling of a Cellular Electric Microenvironment

Published on: May 18, 2021

3.4K
Using Informational Connectivity to Measure the Synchronous Emergence of fMRI Multi-voxel Information Across Time
07:12

Using Informational Connectivity to Measure the Synchronous Emergence of fMRI Multi-voxel Information Across Time

Published on: July 1, 2014

12.3K

Related Experiment Videos

Last Updated: Jun 30, 2025

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
08:47

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation

Published on: January 12, 2024

1.5K
Finite Element Modelling of a Cellular Electric Microenvironment
08:23

Finite Element Modelling of a Cellular Electric Microenvironment

Published on: May 18, 2021

3.4K
Using Informational Connectivity to Measure the Synchronous Emergence of fMRI Multi-voxel Information Across Time
07:12

Using Informational Connectivity to Measure the Synchronous Emergence of fMRI Multi-voxel Information Across Time

Published on: July 1, 2014

12.3K

Area of Science:

  • Cell Biology
  • Structural Biology
  • Biochemistry

Background:

  • The endoplasmic reticulum (ER) membrane protein complex (EMC) is crucial for membrane protein insertion, stabilization, and trafficking.
  • The structural basis and regulatory mechanisms underlying the EMC's multifunctionality are not well understood.

Purpose of the Study:

  • To elucidate the structural basis of the EMC's multifunctionality through cryo-electron microscopy.
  • To investigate the interaction between the EMC and voltage-dependent anion channel (VDAC) proteins.

Main Methods:

  • Cryo-electron microscopy (cryo-EM) was used to determine the structures of the human EMC in apo- and VDAC-bound states.
  • Structural analysis focused on identifying key functional elements and interaction interfaces.

Main Results:

  • High-resolution cryo-EM structures of human EMC in apo- and VDAC-bound states were obtained.
  • A conserved interaction between EMC and VDAC at mitochondria-ER contact sites was identified.
  • A gating plug within the EMC's hydrophilic vestibule was discovered, showing conformational changes upon VDAC binding.

Conclusions:

  • The EMC's gating plug likely regulates its functions by modulating the hydrophilic vestibule.
  • The EMC may not function as an insertase when bound to VDAC1, suggesting context-dependent roles.
  • These findings provide structural insights into the EMC's complex roles in membrane protein biogenesis.