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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Related Experiment Video

Updated: Jun 30, 2025

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

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Biosafety aspects of RNAi-based pests control.

Yimeng Chen1, Kristof De Schutter1

  • 1Molecular Entomology Lab, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Pest Management Science
|March 23, 2024
PubMed
Summary

RNA interference (RNAi) offers sustainable pest management by silencing pest genes. Further research is needed to address RNAi

Keywords:
RNAiRNAi‐based pesticidesbiosafetyhost‐induced gene silencing (HIGS)risk assessmentspray‐induced gene silencing (SIGS)

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • Classical chemical pesticides cause environmental and health issues.
  • RNA interference (RNAi) is a natural gene regulation mechanism.
  • RNAi presents a sustainable alternative for pest control.

Purpose of the Study:

  • To explore RNAi as a novel pest management strategy.
  • To analyze the potential risks and challenges of RNAi applications.
  • To emphasize the need for understanding RNAi mechanisms and safety.

Main Methods:

  • Review of RNA interference mechanisms in pest control.
  • Analysis of potential risks: dsRNA stability, off-target effects, non-target organism safety.
  • Discussion of application challenges and mitigation strategies.

Main Results:

  • RNAi can effectively silence pest-specific genes.
  • Identified challenges include dsRNA stability and off-target effects.
  • Non-target organism safety and application hurdles require further investigation.

Conclusions:

  • RNAi holds significant promise for eco-friendly pest management.
  • Mitigating risks requires a deeper understanding of RNAi molecular mechanisms.
  • Further research is crucial for safe and effective RNAi implementation in agriculture.