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Identification of Potential Hub Genes Related to Acute Pancreatitis and Chronic Pancreatitis via Integrated Bioinformatics Analysis and In Vitro Analysis

  • 0School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China.
Molecular Biotechnology +

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March 23, 2024

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March 23, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study reveals key genes and the Th17 signaling pathway driving the progression from acute pancreatitis to chronic pancreatitis. Findings offer new therapeutic targets for pancreatitis treatment.

Area Of Science

  • Molecular Biology
  • Bioinformatics
  • Gastroenterology

Background

  • Acute pancreatitis (AP) and chronic pancreatitis (CP) are typically viewed as distinct conditions.
  • Emerging evidence suggests a potential link between AP and CP, but the underlying mechanisms remain unclear.

Purpose Of The Study

  • To elucidate the critical signaling pathways and genes involved in the progression from AP to CP using bioinformatics analysis.
  • To identify potential therapeutic targets for pancreatitis.

Main Methods

  • Bioinformatic analysis of murine transcriptomes.
  • Differential gene expression analysis using R and R/Bioconductor.
  • Gene network analysis (STRING), functional enrichment (Metascape), and hub gene screening (Cytoscape).
  • Validation of hub gene mRNA levels via qRT-PCR in AP and CP mouse models.

Main Results

  • Identified key hub genes: Jun, Cd44, Epcam, Spp1, Anxa2, Hsp90aa1, and Cd9, crucial for AP to CP progression.
  • These genes are significantly enriched in the T-helper 17 (Th17) signaling pathway.
  • Up-regulation of these hub genes was confirmed in both AP and CP mouse models.

Conclusions

  • The Th17 signaling pathway plays a significant role in the transition from AP to CP.
  • The identified hub genes (Jun, Cd44, Epcam, Spp1, Anxa2, Hsp90aa1, Cd9) are critical drivers of pancreatitis progression.
  • This research provides molecular insights and potential therapeutic targets for pancreatitis.

Keywords:

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