Fluorogenic polymethine dyes by intramolecular cyclization
Related Experiment Video
Updated: Jun 30, 2025

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
Published on: June 10, 2021
Annabell Martin1, Pablo Rivera-Fuentes2
1Department of Chemistry, University of Zurich, Zurich, Switzerland; École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland.
Abstract:
Fluorescence imaging plays a pivotal role in the study of biological processes, and cell-permeable fluorogenic dyes are crucial to visualize intracellular structures with high specificity. Polymethine dyes are vitally important fluorophores in single-molecule localization microscopy and in vivo imaging, but their use in live cells has been limited by high background fluorescence and low membrane permeability. In this review, we summarize recent advances in the development of fluorogenic polymethine dyes via intramolecular cyclization. Finally, we offer an outlook on the prospects of fluorogenic polymethine dyes for bioimaging.
More Related Videos
12:07Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
Published on: April 1, 2013
06:55Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
Published on: September 26, 2016
Related Concept Videos
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Electrophilic Aromatic Substitution: Fluorination and Iodination of Benzene
Photoluminescence: Applications
Cationic Chain-Growth Polymerization: Mechanism
Variables Affecting Phosphorescence and Fluorescence
Cycloaddition Reactions: Overview