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Updated: Jul 8, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Green immobilization: Enhancing enzyme stability and reusability on eco-friendly support
Vesen Atiroğlu1, Atheer Atiroğlu1, Ahmed Atiroğlu2
1Sakarya University, Biomedical, Magnetic and Semiconductor Materials Application and Research Center (BIMAS-RC), 54050, Sakarya, Turkey; Sakarya University, Biomaterials, Energy, Photocatalysis, EnzymeTechnology, Nan &Advanced Materials, Additive Manufacturing, Environmental Applications, and Sustainability Research & Development Group (BIOENAMS R & D Group), 54050, Sakarya, Turkey.
Abstract:
In the current years of heightened focus on green chemistry and sustainable materials, this study delves into the untapped potential of hyaluronic acid (HA), chitin, and chitosan-prominent polysaccharides for groundbreaking applications. The primary aim is to effectively immobilize catalase enzymes onto matrices composed of chitosan, chitin, HA/chitin, and HA/chitosan. The rigorous investigation covers a spectrum of structural enhancements encompassing pH and temperature stability, thermal resilience, half-life extension, storage durability, reusability, and comprehensive FTIR analyses of the catalase immobilization. Notably, catalase activity demonstrated remarkable resilience on HA/chitin and HA/chitosan matrices, maintaining 73.80% and 79.55% efficacy even after 25 cycles. The introduction of covalent cross-linking between catalase and HA/chitin or HA/chitosan, facilitated by a cross-linking agent, significantly amplified stability and recycling efficiency. Consequently, the immobilized catalase showcases substantial promise across a spectrum of industrial applications, spanning from food and detergent production to bioremediation and diverse commercial processes. This underscores its pivotal role as a versatile and invaluable innovation in the realm of sustainable technologies.
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