Profile of uterine flush lipid mediators in cows with subclinical endometritis: pilot study

Subclinical endometritis affects reproductive outcomes and causes economic losses in dairy cows, thus, it is important to understand disease progression mechanisms and develop diagnostic procedures for better disease management. We measured the levels of 146 lipid mediators in uterine flush samples using lipid chromatography-mass spectrometry. We detected 25 lipid mediators in the uterine flush of both the control and subclinical endometritis cows; 15 of the 25 lipid mediators were AA-derived metabolites. Among the AA-derived metabolites, cyclooxygenase (COX)-generated mediators were the most abundant. Specifically, levels of 11β-13,14-dihydro-15-keto prostaglandin (PG) F_2α, PGE₂, PGA₂, 13-hydroxyoctadecadienoic acid, and PGD₁ were elevated in all the cows with subclinical endometritis. This study may provide new insights for the management of subclinical bovine endometritis.