PERP May Affect the Prognosis of Lung Adenocarcinoma by Inhibiting Apoptosis

Area of Science:

• Oncology
• Immunology
• Molecular Biology

Background:

• Peripheral myelin protein (PERP) is a potential therapeutic target in cancer.
• The role of PERP in the lung cancer tumor microenvironment (TME) and its association with immune cell infiltration remain understudied.

Purpose of the Study:

• To investigate the association between PERP expression and clinical characteristics in lung adenocarcinoma (LUAD).
• To explore the relationship between PERP expression and immune cell infiltration in LUAD.
• To elucidate the functional role of PERP in LUAD progression and metastasis.

Main Methods:

• Analysis of TCGA database for PERP expression and clinical characteristics.
• Cox regression and Kaplan-Meier analyses for overall survival.
• TIMER and GEPIA databases for immune cell infiltration analysis.
• GO and KEGG enrichment analyses for coexpressed genes.
• In vitro (RT-PCR, Western blot, CCK-8, IHC, wound healing) and in vivo (mouse model) validation of PERP function.

Main Results:

• PERP expression is significantly higher in LUAD tissues and correlates with clinical characteristics.
• PERP expression is an independent prognostic factor for LUAD patients.
• PERP may regulate B-cell infiltration in LUAD, influencing patient prognosis.
• PERP inhibits LUAD cell proliferation and metastasis by inducing apoptosis.

Conclusions:

• PERP plays a significant role in the tumor microenvironment of lung adenocarcinoma.
• PERP affects LUAD prognosis through its association with immune cell infiltration and induction of apoptosis.