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Related Experiment Video

Updated: May 13, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Spatial High-resolution Analysis of Gene Expression Levels in Tendons.

Steffany Villaseñor¹, Mor Grinstein²

¹Center for Regenerative Medicine, Department of Orthopedic Surgery, Massachusetts General Hospital, Harvard Medical School.

Journal of Visualized Experiments : Jove

|March 25, 2024

Summary

Spatial transcriptomics methods are now available for analyzing gene expression in tendons, overcoming challenges posed by their dense matrix. These RNA spatial tools enable single-cell level analysis for better tendon research.

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Last Updated: May 13, 2026

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Published on: July 6, 2022

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Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Author Spotlight: Advancing Tendon Research by Developing Mouse Assembloids to Understand Cellular Mechanisms

Published on: March 22, 2024

Area of Science:

Biotechnology
Molecular Biology
Tissue Engineering

Background:

High-resolution transcriptomics protocols exist but struggle with matrix-rich tissues like tendons due to low cell numbers and RNA content.
Spatial analysis of gene expression is crucial for understanding cell populations and validating single-cell RNA sequencing (scRNA-seq) data in tendons.

Purpose of the Study:

To present novel in situ hybridization (ISH) assays for spatial gene expression analysis in tendon histological sections.
To detail methods for tissue preparation, signal amplification, and probe design for sensitive and specific RNA detection at the single-cell level.

Main Methods:

Focus on preparing tendon tissue for various assays.
Describe methods for amplifying target-specific signals with high sensitivity and specificity, minimizing background noise.
Discuss specific permeabilization techniques, probe designs, and signal amplification strategies.

Main Results:

The described methods enable sensitive, specific detection of RNA targets at the single-cell level within intact cells.
These techniques facilitate the molecular characterization of tendon cells in diverse populations (young, aged, animal models, human).

Conclusions:

Novel ISH assays provide a powerful tool for spatial gene expression analysis in tendons.
These methods can advance tendon research and be applied to other matrix-rich tissues like bone, cartilage, and ligaments.