Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Long-term sero-epidemiological trends of Nine respiratory pathogens in a predominantly pediatric population in Southwest China: a multicenter retrospective study (2019-2023).

BMC infectious diseases·2026

Same author

Intra-seafood strain diversity and temporal dynamics of Vibrio parahaemolyticus in a retail stall.

International journal of food microbiology·2026

Same author

Investigation the implementation of evidence-based protocols for cannulation in Arteriovenous Fistula: a mixed methods study.

BMC nephrology·2026

Same author

Correction: Effects of exercise regimens on balance ability in older patients with osteoporosis: a systematic review and Bayesian network meta-analysis of randomized controlled trials.

Frontiers in physiology·2026

Same author

Influence of solvent effect, temperature, and pressure on the aggregation behavior of island model asphaltene: a molecular dynamics study.

RSC advances·2026

Same author

Risk Factors for Sporadic Non-Typhoidal <i>Salmonella</i> Infection Among Children Under Five Years of Age in an Urban Area of Kunming City: A Matched Case-Control Study.

Foodborne pathogens and disease·2026

Search research articles

Related Experiment Video

Updated: Jun 29, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

Exploring transient global transcriptional changes induced by ascorbic acid revealed via atKAS-seq profiling.

Xiangyue Liu¹, Weizhi He¹, Lulu Hu²

¹Cancer Institute, Fudan University Shanghai Cancer Center, Institutes of Biomedical Sciences, Shanghai Key Laboratory of Medical Epigenetics, International Co-Laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Shanghai Medical College of Fudan University, Shanghai, 200032, China.

Functional & Integrative Genomics

|March 25, 2024

Summary

A new method, adapter-tagged Kethoxal-assisted single-stranded DNA sequencing (atKAS-seq), efficiently maps DNA. This cost-effective technique analyzes transcription dynamics and enhancer activity, even with low sample input.

Keywords:

Adapter tagging Ascorbic acid AtKAS-seq Transcription dynamics

More Related Videos

ATAC-Seq Optimization for Cancer Epigenetics Research

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jun 29, 2025

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

ATAC-Seq Optimization for Cancer Epigenetics Research

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

Transcription creates dynamic single-stranded DNA (ssDNA) regions called transcription bubbles.
Kethoxal-assisted single-stranded DNA sequencing (KAS-seq) maps guanines in ssDNA genome-wide.
The original KAS-seq method has high costs and requires specific library kits.

Purpose of the Study:

To optimize KAS-seq for cost-efficiency and broader accessibility.
To introduce adapter-tagged KAS-seq (atKAS-seq) for streamlined ssDNA analysis.
To investigate epigenetic changes induced by ascorbic acid (ASC) using atKAS-seq.

Main Methods:

Developed adapter-tagged KAS-seq (atKAS-seq) with complementary strand synthesis and random N9 tagging.
Integrated sequencing adapters via a novel tagging strategy.
Applied atKAS-seq to study effects of short-term, high-dose ascorbic acid treatment.

Main Results:

atKAS-seq provides a cost-efficient, low-input alternative to original KAS-seq.
The method enables rapid and precise analysis of transcription dynamics.
atKAS-seq concurrently maps enhancer activities, revealing insights into ASC-induced epigenetic changes.

Conclusions:

atKAS-seq is a versatile tool for probing complex genomic regulatory mechanisms.
The optimized method enhances the study of transcription and enhancer function.
atKAS-seq facilitates accessible, high-resolution genomic analyses.