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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Jun 29, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Obtaining Detailed Phage Transcriptomes Using ONT-Cappable-Seq.

Leena Putzeys¹, Danish Intizar¹, Rob Lavigne²

¹Laboratory of Gene Technology, Department of Biosystems, KU Leuven, Leuven, Belgium.

Methods in Molecular Biology (Clifton, N.J.)

|March 25, 2024

Summary

ONT-cappable-seq enables detailed phage transcriptomics using nanopore sequencing. This method provides a comprehensive overview of phage transcription, improving molecular phage biology understanding and engineering potential.

Keywords:

Bacteriophage Nanopore sequencing Operons Promoter identification Terminators Transcriptomics

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Last Updated: Jun 29, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

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Published on: June 11, 2015

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Published on: January 10, 2019

Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Bacteriophage transcription maps are underexplored, hindering molecular phage biology insights.
Limited understanding restricts phage exploitation and engineering applications.

Approach:

Introduces ONT-cappable-seq for accessible phage transcriptomics on nanopore sequencing platforms.
Primary transcripts are specifically capped, enriched, and prepared for long-read sequencing.
Enables end-to-end sequencing of unprocessed transcripts, covering both phage and host genomes.

Key Points:

Provides a more affordable and detailed overview of transcriptional features than traditional RNA-seq.
Offers insight into phage-host operons through comprehensive transcript sequencing.
Facilitates rapid identification of key transcriptional features like start and stop sites.

Conclusions:

ONT-cappable-seq offers a powerful tool for detailed phage transcription analysis.
Enhances understanding of molecular phage biology and engineering capabilities.
Paves the way for a comprehensive overview of bacteriophage transcription.