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Updated: Jun 29, 2025
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
MicroRNA-30e-3p regulates the inflammatory response by targeting the gimap8 gene in Ctenopharyngodon idella kidney
Lingli Xie1, Biao Du1, Hongyan Yu1
1Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
Abstract:
Recent studies have increasingly linked miRNAs with the modulation of inflammatory responses and immunosuppressive activities. This investigation reveals that mir-30e-3p selectively binds to and modulates gimap8, as demonstrated by luciferase reporter assays and qPCR analyses. Upon LPS stimulation of CIK cells, mir-30e-3p expression was notably elevated, inversely correlating with a decrease in gimap8 mRNA levels. Overexpression of mir-30e-3p attenuated the mRNA levels of pro-inflammatory cytokines beyond the effect of LPS alone, suggesting a regulatory role of mir-30e-3p in inflammation mediated by the gimap8 gene. These insights contribute to our understanding of the complex mechanisms governing inflammatory and immune responses.
Insights
MicroRNAs (miRNAs) regulate inflammation. This study shows microRNA-30e-3p (mir-30e-3p) targets gimap8, reducing inflammatory cytokine expression and impacting immune responses.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are increasingly recognized for their roles in regulating inflammatory and immune responses.
- Specific miRNAs and their target genes involved in these processes are still being elucidated.
Purpose of the Study:
- To investigate the role of mir-30e-3p in modulating inflammatory responses.
- To identify the target gene of mir-30e-3p involved in inflammation.
- To understand the regulatory mechanism of mir-30e-3p in immune cells.
Main Methods:
- Luciferase reporter assays to confirm target binding.
- Quantitative Polymerase Chain Reaction (qPCR) to measure gene and miRNA expression.
- Lipopolysaccharide (LPS) stimulation of Cytokine-Induced Killer (CIK) cells.
- Overexpression of mir-30e-3p in CIK cells.
Main Results:
- Mir-30e-3p was found to selectively bind and modulate the gimap8 gene.
- LPS stimulation led to increased mir-30e-3p expression and decreased gimap8 mRNA levels.
- Overexpression of mir-30e-3p reduced pro-inflammatory cytokine mRNA levels.
Conclusions:
- Mir-30e-3p plays a regulatory role in inflammation, potentially mediated through the gimap8 gene.
- These findings enhance the understanding of miRNA-mediated regulation in inflammatory and immune pathways.
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