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Updated: Jun 29, 2025

The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Split Proteins and Reassembly Modules for Biological Applications
Jieun Bae1, Jonghoon Kim2, Jongdoo Choi1
1Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan, 46241, Republic of Korea.
Abstract:
Split systems, modular entities enabling controlled biological processes, have become instrumental in biological research. This review highlights their utility across applications like gene regulation, protein interaction identification, and biosensor development. Covering significant progress over the last decade, it revisits traditional split proteins such as GFP, luciferase, and inteins, and explores advancements in technologies like Cas proteins and base editors. We also examine reassembly modules and their applications in diverse fields, from gene regulation to therapeutic innovation. This review offers a comprehensive perspective on the recent evolution of split systems in biological research.
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