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Updated: Jun 29, 2025
12:59
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 26, 2013
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Molecular and Structural Insights into Buffalo Interleukin-17A.
Savita Budania1, Surinder Kumar Kadian1, Karuppiah Kanagarajadurai2
1Department of Veterinary Microbiology and Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar, Haryana, India.
Summary
Researchers cloned and analyzed the buffalo Interleukin-17A (IL-17A) gene, finding it highly similar to cattle IL-17A. This recombinant buffalo IL-17A protein shows potential as an immunobiological reagent for diagnostics and therapeutics.
Area of Science:
- Immunology
- Molecular Biology
- Bioinformatics
Background:
- Interleukin-17A (IL-17A) is a crucial pro-inflammatory cytokine involved in immune responses against pathogens and autoimmune diseases.
- IL-17A also provides protection against bacterial and fungal infections in the gastro-intestinal tract and respiratory mucosa.
- Limited molecular data exists for buffalo IL-17A, despite buffalo's significance in milk production.
Purpose of the Study:
- To clone, express, and analyze the Indian Murrah buffalo IL-17A gene.
- To computationally investigate the sequence, structure, and evolutionary relationships of buffalo IL-17A (buIL-17A).
- To assess the potential of recombinant buIL-17A protein as an immunobiological reagent.
Main Methods:
- Cloning of the buffalo IL-17A gene into a prokaryotic expression vector (pET-28a).
- Expression, purification, and characterization of the recombinant buffalo IL-17A protein.
- Bioinformatic analysis of the buIL-17A sequence, structure, and evolutionary context.
Main Results:
- The coding sequence of buIL-17A without a signal peptide is 132 amino acids long, similar to cattle.
- Sequence identity between buffalo and cattle IL-17A is 99%, with a single amino acid substitution.
- Successful expression and purification of recombinant buIL-17A protein were achieved.
Conclusions:
- The cloned and expressed buffalo IL-17A protein is highly conserved compared to cattle.
- Recombinant buIL-17A protein holds potential as a valuable immunobiological reagent.
- This study provides foundational molecular data for buffalo IL-17A, applicable in diagnostics and therapeutics.
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