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Heavy metal ion-nucleic acid interaction.

S H Kim, W C Shin, R W Warrant

    Methods in Enzymology
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Heavy atoms bind nucleic acids via coordination bonds, forming stable crystals. These heavy atoms target specific sites on the nucleic acid backbone and bases, including phosphates and nitrogen atoms like guanine N-7.

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    Area of Science:

    • Biochemistry
    • Crystallography
    • Structural Biology

    Background:

    • Heavy atoms are crucial for determining nucleic acid structures through derivative crystallography.
    • Successful heavy atom derivatives rely on specific coordination bonding and stabilization by hydrogen bonds.

    Purpose of the Study:

    • To summarize known coordination sites of heavy atoms on nucleic acids.
    • To provide a reference for selecting appropriate heavy atoms for crystallographic studies.

    Main Methods:

    • Analysis of existing crystallographic data on heavy atom derivatives of nucleic acids.
    • Compilation of coordination sites based on literature and structural analysis.

    Main Results:

    • Lanthanide ions coordinate to phosphate oxygens.

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  • Guanine N-7 is a common binding site for various metal ions (Os, Pt, Co, Mn).
  • Uracil O-4 and adenine N-7/cytosine N-4 are also identified as binding sites.

    • Conclusions:

    • Specific heavy atoms exhibit predictable binding preferences on nucleic acid structures.
    • Understanding these coordination sites is essential for successful structure determination of nucleic acids.