Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K
Channel Rhodopsins01:11

Channel Rhodopsins

2.6K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.6K
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

1.4K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
1.4K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

3.4K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.4K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

4.6K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
4.6K
Types of Signaling Molecules01:32

Types of Signaling Molecules

10.3K
In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small peptides, nucleotides, steroids, fatty acid derivatives, and dissolved gases such as nitric oxide. Some signaling molecules diffuse through the plasma membrane to act locally between neighboring cells or travel long distances. Others remain attached to the cell surface, transmitting information to other cells only when they make contact. In some...
10.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collective bacterial condensation is fundamentally constrained by the emergence of active turbulence.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Single-array measurements reveal non-uniform, mosaic-like chemosensory arrays in bacteria.

Nature communications·2025
Same author

The structural logic of dynamic signaling in the Escherichia coli serine chemoreceptor.

Protein science : a publication of the Protein Society·2024
Same author

Collective condensation and auto-aggregation of Escherichia coli in uniform acidic environments.

Communications biology·2024
Same author

The Structural Logic of Dynamic Signaling in the <i>Escherichia coli</i> Serine Chemoreceptor.

bioRxiv : the preprint server for biology·2024
Same author

The Chemoreceptor Sensory Adaptation System Produces Coordinated Reversals of the Flagellar Motors on an Escherichia coli Cell.

Journal of bacteriology·2022
Same journal

In This Issue.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Dolphins use names to remember whom to avoid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Small but mighty: The outsized role of small water bodies in the global carbon cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Functional traits produce conditional outcomes in different community contexts.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2025

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

18.0K

Signal integration in chemoreceptor complexes.

Moriah Koler1, John S Parkinson2, Ady Vaknin1

  • 1The Racah Institute of Physics, The Hebrew University, Jerusalem 91904, Israel.

Proceedings of the National Academy of Sciences of the United States of America
|March 26, 2024
PubMed
Summary
This summary is machine-generated.

Bacteria use complex chemoreceptor arrays to navigate. This study reveals that individual receptor trimers, not just binary switches, integrate signals, enabling sophisticated environmental sensing for motile bacteria.

Keywords:
cell signalingchemotaxisreceptor arraysignal integration

More Related Videos

Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes
06:32

Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes

Published on: November 17, 2023

1.3K
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

7.0K

Related Experiment Videos

Last Updated: Jun 29, 2025

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

18.0K
Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes
06:32

Author Spotlight: Visualizing Olfactory Receptor Expression in Mosquitoes

Published on: November 17, 2023

1.3K
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

7.0K

Area of Science:

  • Microbiology
  • Biophysics
  • Molecular Biology

Background:

  • Motile bacteria possess extensive chemoreceptor arrays for environmental sensing and directed movement.
  • These arrays are composed of core signaling complexes, each containing receptor trimers and a kinase enzyme.
  • The precise molecular mechanisms of signal processing within these arrays are not fully understood.

Purpose of the Study:

  • To investigate the signaling properties of non-networked core complexes in live bacterial cells.
  • To elucidate the conformational and kinase control responses to stimuli and molecular perturbations.
  • To challenge the binary two-state model of individual receptor function.

Main Methods:

  • Live-cell imaging of bacterial chemoreceptor signaling.
  • Analysis of conformational changes in response to attractant stimuli.
  • Perturbation studies using output-biasing lesions at specific receptor locations.
  • Investigation of inter-receptor communication via trimer-contact domains.

Main Results:

  • Conformational coupling between ligand-binding and kinase-control domains is only moderate, not absolute.
  • Neighboring receptors communicate through trimer-contact domains, promoting similar signaling states.
  • Individual core complexes exhibit significant signal integration capabilities.

Conclusions:

  • Bacterial chemoreceptor signaling is more nuanced than a simple binary switch model.
  • Signal integration occurs within individual core complexes, not solely through large-scale array networks.
  • This provides a refined understanding of how bacteria process environmental information for motility.