Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
MicroRNAs01:22

MicroRNAs

21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K
Riboswitches01:56

Riboswitches

8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

m<sup>6</sup>A in RNA: a key regulator of brain development, function and disease.

Nature reviews. Neuroscience·2026
Same author

Transcript architecture sets the m <sup>6</sup> A landscape: CSTF2 and CSTF2T reshape m <sup>6</sup> A through cleavage-dependent and -independent mechanisms.

bioRxiv : the preprint server for biology·2026
Same author

The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpoint.

Cell reports·2025
Same author

Nucleotide-resolution Mapping of RNA N6-Methyladenosine (m6A) modifications and comprehensive analysis of global polyadenylation events in mRNA 3' end processing in malaria pathogen <i>Plasmodium falciparum</i>.

bioRxiv : the preprint server for biology·2025
Same author

Simultaneous profiling of the RNA targets of two RNA-binding proteins using TRIBE-STAMP.

Methods in enzymology·2024
Same author

Single-cell m<sup>6</sup>A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation.

Nature neuroscience·2024
Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026
Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026
Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026
Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026
Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.7K

mRNA epitranscriptomics.

Kate D Meyer1, Tao Pan2

  • 1Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710, USA taopan@uchicago.edu kate.meyer@duke.edu.

RNA (New York, N.Y.)
|March 26, 2024
PubMed
Summary
This summary is machine-generated.

Epitranscriptomics involves chemical modifications to RNAs, influencing diverse biological functions like transcription and translation. This research area, focusing on messenger RNA (mRNA), aims to advance understanding and applications in human health.

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.6K
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

13.5K

Related Experiment Videos

Last Updated: Jun 29, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

11.7K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.6K
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

13.5K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Epitranscriptomics encompasses diverse chemical modifications of RNA molecules.
  • These RNA modifications exhibit cell-type and cell-state specificity, impacting biological functions.
  • RNA modifications play crucial roles in regulating transcription, translation, chromatin maintenance, and immune responses.

Purpose of the Study:

  • To present a comprehensive overview of epitranscriptomics research, with a specific emphasis on messenger RNA (mRNA).
  • To consolidate expert perspectives on the past, present, and future trajectory of the field.
  • To foster discussions and debates for advancing epitranscriptomics in basic research and clinical applications.

Main Methods:

  • Review of existing literature and expert opinions.
  • Synthesis of current knowledge and future directions in RNA modification research.
  • Focus on messenger RNA (mRNA) modifications and their functional implications.

Main Results:

  • RNA modifications are integral to a wide spectrum of biological processes.
  • The field of epitranscriptomics is rapidly evolving, with significant implications for understanding gene expression.
  • Expert insights highlight the potential of epitranscriptomics in human health and disease.

Conclusions:

  • Epitranscriptomics is a dynamic field with broad biological relevance.
  • Further research and expert collaboration are essential to unlock the full potential of RNA modifications.
  • Advancements in epitranscriptomics promise novel applications in medicine and biotechnology.