Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

10.2K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Singlár et al. Revealing the Specific Contributions of Mitochondrial CB<sub>1</sub> Receptors to the Overall Function of Skeletal Muscle in Mice. <i>Cells</i> 2025, <i>14</i>, 1517.

Cells·2026
Same author

Maternal Diet, Lifestyle Factors, and Gestational Weight Gain: A Single-Center Case-Control Study in Hungary.

Nutrients·2026
Same author

Cluster-Defined Metabolic Heterogeneity and Longitudinal Effects of Allopurinol and SGLT2 Inhibitors in Hyperuricemic Type 2 Diabetes.

Medical sciences (Basel, Switzerland)·2026
Same author

Non-destructive estimation of maize carotenoids using reflectance-based spectral indices.

Frontiers in plant science·2026
Same author

Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.

Antioxidants (Basel, Switzerland)·2026
Same author

Role of Septin7 in mitochondrial dynamics and oxidative metabolism in C2C12 skeletal muscle cells.

The Journal of physiology·2026

Related Experiment Video

Updated: Jun 29, 2025

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
04:01

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

702

Microcirculation-driven mitochondrion dysfunction during the progression of experimental sepsis.

Roland Fejes1, Attila Rutai1, László Juhász1

  • 1Institute of Surgical Research, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, 6720, Hungary.

Scientific Reports
|March 27, 2024
PubMed
Summary

Sepsis-induced organ failure is preceded by impaired microcirculation, not mitochondrial dysfunction. Early detection of splanchnic microcirculatory changes can predict organ failure development.

More Related Videos

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
05:28

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Published on: December 9, 2022

3.5K
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

39.5K

Related Experiment Videos

Last Updated: Jun 29, 2025

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
04:01

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

702
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
05:28

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Published on: December 9, 2022

3.5K
Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
09:27

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

Published on: March 23, 2015

39.5K

Area of Science:

  • Physiology
  • Pathophysiology
  • Mitochondrial Biology

Background:

  • Sepsis is characterized by hemodynamic alterations and impaired mitochondrial respiration.
  • The relationship between microcirculatory function and mitochondrial respiration in sepsis remains unclear.
  • Understanding this interplay is crucial for predicting multi-organ failure (MOF).

Purpose of the Study:

  • To investigate the temporal relationship between splanchnic microcirculation and mitochondrial function during sepsis.
  • To identify predictors of MOF in a rodent model of intraabdominal sepsis.
  • To explore the role of mitochondrial outer membrane damage in sepsis-induced organ dysfunction.

Main Methods:

  • Intraabdominal sepsis was induced in rats using fecal peritonitis.
  • Multi-organ failure (MOF) was assessed using rat-specific organ failure assessment (ROFA) scores at various time points.
  • Ileal microcirculation (proportion of perfused microvessels (PPV), microvascular flow index (MFI), heterogeneity index (HI)) was measured using intravital video microscopy.
  • Mitochondrial respiration (OxPhos) and outer membrane (mtOM) damage were analyzed using high-resolution respirometry.

Main Results:

  • MOF progression correlated with increased ROFA scores from 16 to 28 hours post-induction.
  • Microcirculatory parameters showed a parallel time course with MOF.
  • Mitochondrial dysfunction, indicated by mtOM damage, occurred with a 4-hour delay compared to microcirculatory changes.
  • PPV and mtOM damage were significant predictors of MOF, outperforming HI and OxPhos.
  • Lactate levels also demonstrated high diagnostic value for predicting MOF.

Conclusions:

  • Insufficient splanchnic microcirculation appears to be an early predictor of MOF in sepsis.
  • Microcirculatory impairment precedes mitochondrial dysfunction in this sepsis model.
  • The findings suggest compensatory mechanisms within mitochondria, with subpopulations exhibiting varied sensitivity to septic insults.