Immunostaining for Epigenetic Modifications in Fagopyrum Calli
Alicja Tomasiak1, Katarzyna Sala1, Agnieszka Braszewska2
1Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.
Methods in Molecular Biology (Clifton, N.J.)
|March 27, 2024
Abstract
None:
Immunostaining is a well-established technique for identifying specific proteins in tissue samples with specific antibodies to identify a single target protein. It is commonly used in research and provides information about cellular localization and protein expression levels. This chapter describes a detailed protocol for immunostaining fixed Fagopyrum calli embedded in Steedman's wax using nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines and DNA methylation.


