Related Experiment Video
Updated: Jul 5, 2026

An Improved Method of RNA Isolation from Loblolly Pine P. taeda L. and Other Conifer Species
Published on: February 22, 2010
Two novel endophytic Tolypocladium species identified from native pines in south Florida
J M Soares1,2, E Karlsen-Ayala1,3, C A Salvador-Montoya1
1Tropical Research and Education Center, Department of Plant Pathology, University of Florida, Homestead, FL 33031, USA.
Abstract:
This study investigated the incidence and diversity of Tolypocladium within trunks of south Florida slash pines (Pinus densa). Thirty-five isolates were recovered from trunk tissue including living phloem, cambium, and sapwood. Two novel species of Tolypocladium (T. subtropicale and T. trecense) are described here based on morphological and molecular analysis of concatenated LSU, ITS, tef-1, tub, and RPB1 sequences. Our findings expand our understanding of the distribution, diversity, and ecology of this genus and confirm that it is widely spread as an endophyte across ecosystems and hosts. Strains collected in this survey will be used in future bioassays to determine their potential ecological roles as mycoparasites or entomopathogens. Citation: Soares JM, Karlsen-Ayala E, Salvador-Montoya CA, Gazis R (2023). Two novel endophytic Tolypocladium species identified from native pines in south Florida. Fungal Systematics and Evolution 11: 51-61. doi: 10.3114/fuse.2023.11.04.
Related Concept Videos
Epiphytes, Parasites, and Carnivores
Bacterial Phylum Tenericutes
Fungal Group Zygomycota
Fungal Phylum Microsporidia
Fungal Phylum Basidiomycota
Diversity of Protists II

