Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unravelling the key factors governing O<sub>2</sub> evolution upon charging a reversible LiOH-based nonaqueous Li | |O<sub>2</sub> battery.

Nature communications·2026
Same author

Inorganic Ionic Polymerization-Anchored Polymer Networks for Ultrastrong and Ultratough Eutectogels.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Optimizing nitrogen and maize plant density to improve competitive advantage and fertilizer use efficiency in maize soybean intercropping in Northwest China.

Scientific reports·2026
Same author

<i>De novo NFKBIA</i> variants within the N-terminal hotspot: consistent immunophenotype and divergent clinical presentations.

Frontiers in immunology·2026
Same author

Ultrasound-guided erector spinae plane block versus serratus anterior plane block for analgesia and respiratory function in patients with multiple rib fractures: a large-sample, single-center, randomized, double-blind, controlled trial.

Frontiers in medicine·2026
Same author

A novel compound heterozygous NBAS variant with HLH and multisystem involvement: expanding the clinical spectrum and literature review.

Frontiers in immunology·2026

Related Experiment Video

Updated: Jun 29, 2025

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes
11:59

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

Published on: May 19, 2023

2.2K

A pH-response-based fluorescent probe for detecting the mitophagy process by tracing changes in colocalization

Nongyi Hao1, Zekun Jiang1, Lina Zhou1

  • 1School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, P. R. China. daixiaoyu0111@163.com.

Analytical Methods : Advancing Methods and Applications
|March 27, 2024
PubMed
Summary

Researchers developed a pH-responsive fluorescent probe (MP) to monitor mitophagy, the process of clearing damaged mitochondria. This new tool enables real-time tracking of mitophagy in living cells, aiding in understanding metabolic disorders.

More Related Videos

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

4.4K
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

14.8K

Related Experiment Videos

Last Updated: Jun 29, 2025

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes
11:59

Author Spotlight: Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

Published on: May 19, 2023

2.2K
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

4.4K
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
09:13

Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima

Published on: August 12, 2018

14.8K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • Mitochondria are crucial for cellular energy metabolism and regulation.
  • Maintaining mitochondrial quality and quantity is essential for cellular health.
  • Mitophagy, a lysosome-dependent process for clearing dysfunctional mitochondria, is vital, and its dysregulation is linked to metabolic disorders.

Purpose of the Study:

  • To develop a novel molecular tool for real-time monitoring of mitophagy in living cells.
  • To create a pH-responsive fluorescent probe for sensitive and selective detection of the mitophagy process.

Main Methods:

  • Development of a positively charged, pH-responsive fluorescent probe (MP).
  • Spectral testing for probe selectivity and sensitivity.
  • Real-time tracing and colocalization analysis with Lyso Tracker® Deep Red in living cells.
  • Stimulation of mitophagy using CCCP, starvation, and rapamycin.

Main Results:

  • Probe MP demonstrated excellent pH responsiveness, selectivity, and sensitivity.
  • The probe's positive charge facilitated mitochondrial accumulation in living cells.
  • pH-dependent fluorescence changes were observed in cells treated with varying pH media.
  • A significant real-time increase in the colocalization coefficient between probe MP and Lyso Tracker® Deep Red (0.4 to 0.8) was observed during mitophagy.

Conclusions:

  • Probe MP is a valuable tool for real-time mitophagy tracing.
  • The colocalization coefficient serves as a reliable parameter for monitoring mitophagy.
  • This probe facilitates further exploration of mitophagy dynamics and its role in metabolic disorders.