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Related Experiment Video

Updated: Jun 29, 2025

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
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Fluorometric and Colorimetric Method for SARS-CoV-2 Detection Using Designed Aptamer Display Particles.

Ki Sung Park1, Anna Choi1, Tae-In Park1

  • 1Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea.

Biosensors
|March 27, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed novel aptamer display particles (AdPs) for rapid, low-cost SARS-CoV-2 detection. These AdPs enable sensitive diagnosis using simple fluorometric and colorimetric assays, offering a promising alternative to existing methods.

Keywords:
SARS-CoV-2 aptamerSARS-CoV-2 spike protein-binding aptameraptamer display particlescolorimetric aptasensorfluorometric aptasensorparticle display

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • The COVID-19 pandemic necessitates sensitive and selective diagnostic tools.
  • Current diagnostics often rely on expensive antibodies or enzymes and high-tech equipment.
  • There is a critical need for rapid, low-cost SARS-CoV-2 detection methods.

Purpose of the Study:

  • To develop and validate aptamer display particles (AdPs) for SARS-CoV-2 detection.
  • To create a simple, low-cost diagnostic matrix for SARS-CoV-2.
  • To evaluate the sensitivity and selectivity of AdP-based assays.

Main Methods:

  • Generation of two distinct AdPs (C1-AdP and C4-AdP) displaying specific aptamers.
  • Utilizing particle PCR for fabricating a SARS-CoV-2 detection matrix.
  • Employing fluorometric and colorimetric assays for diagnosis.
  • Designing a sandwich-type assay with AdPs and aptamers targeting SARS-CoV-2 pseudoviruses.

Main Results:

  • AdPs demonstrated high binding affinity to SARS-CoV-2 spike protein, with concentration-dependent fluorescence.
  • Detection of aptamer binding at low concentrations (0.5 nM).
  • Fluorometric assay achieved a detection limit of 3.9 × 10^3 pseudoviruses/mL.
  • Colorimetric assay with amplification reached a detection limit of 1 × 10^1 pseudoviruses/mL over a broad range.

Conclusions:

  • Aptamer display particles offer a versatile platform for SARS-CoV-2 detection.
  • The developed AdP-based assays are sensitive, selective, and potentially low-cost.
  • These findings present a promising advancement in rapid diagnostic technologies for viral pandemics.