Immunogenetic Background of Chronic Lymphoproliferative Disorders in Romanian Patients-Case Control Study

Area of Science:

• Immunogenetics
• Hematology
• Population Genetics

Background:

• Genetic factors significantly influence the development of chronic lymphoproliferative disorders (CLPD).
• Human leukocyte antigen (HLA) genes are critical in the pathogenesis of various diseases.
• Understanding HLA associations in CLPD is crucial for risk assessment and potential therapeutic strategies.

Purpose of the Study:

• To investigate the association between specific HLA alleles and chronic lymphoproliferative disorders in the Romanian population.
• To identify HLA alleles that may predispose to or protect against CLPD in this demographic.

Main Methods:

• High-resolution genotyping of HLA class I (HLA-A/B/C) and class II (HLA-DQB1/DPB1/DRB1) genes was performed.
• Sequence-specific primers (SSP) were utilized for genotyping.
• A cohort of 38 patients with CLPD and 50 healthy controls from Romania were analyzed.

Main Results:

• The study identified significant associations between specific HLA alleles and CLPD.
• HLA-C*02:02 and HLA-C*12:02 alleles were found to have a predisposing role (p = 0.002 for both).
• Protective roles were observed for HLA-A*11:01 (p = 0.01), HLA-B*35:02 (p = 0.037), HLA-B*81:01 (p = 0.037), HLA-C*07:02 (p = 0.036), HLA-DRB1*11:01 (p = 0.021), and HLA-DRB1*13:02 (p = 0.037).

Conclusions:

• HLA-C*02:02 and HLA-C*12:02 alleles are positively associated with CLPD in the Romanian population.
• HLA-DRB1*11:01, HLA-DRB1*13:02, and HLA-B*35:02 alleles exhibit a protective effect against CLPD in this cohort.
• These findings contribute to understanding the genetic landscape of CLPD in Romania and may inform future research and clinical practice.