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Updated: Jun 29, 2025
Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
Published on: June 13, 2010
Rapid detection of SARS-CoV-2 spike protein using a magnetic-assisted electrochemical biosensor based on
Bing Zhao1, Chan-Ru Xiong2, Yao Liu2
1Key Lab of Aerospace Structural Parts Forming Technology and Equipment of Anhui Province, Institute of Industry and Equipment Technology, Hefei University of Technology, Hefei, 230009, PR China; School of Materials Science and Engineering, Hefei University of Technology, Hefei, 230009, PR China.
Abstract:
The outbreak of novel coronavirus pneumonia (COVID-19) in 2019 has garnered widespread attention. The virus exhibits high contagiousness, and in certain cases, it can lead to recurrent infections. Therefore, it is imperative to develop portable, sensitive, and accurate sensors to promptly detect infected individuals, control the virus's transmission, and determine suitable treatment strategies. In this study, we proposed a magnetically-assisted method employing CFO@CS-Au MNP as the substrate material, which was functionalized with human angiotensin-converting enzyme (ACE2) for efficient capture of SARS-CoV-2 spike protein in solution. Subsequently, the captured protein was sensitively detected through differential pulse voltammetry (DPV) electrical analysis. The linear detection range of the labeled GCE/MNP/GA/ACE2/BSA electrochemical sensor is from 1 pg/mL to 10 μg/mL, with a minimum detection limit of 0.15 pg/mL. Furthermore, the fabricated GCE/MNP/GA/ACE2/BSA sensor achieved satisfactory recoveries of SARS-CoV-2 spike protein in saliva and nasal swab samples within 10 min. These results indicate that this magnetically-assisted biosensor has established a solid foundation for the swift on-site detection of COVID-19.
