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NKX2-1 gene is targeted by H19 lncRNA and is found to be overexpressed in benign nodular goiter tissues

Seyed-Morteza Javadirad1

  • 1University of Isfahan, Faculty of Biological Science and Technology, Department of Cell and Molecular Biology and Microbiology, Isfahan, Iran.

Brazilian Journal of Otorhinolaryngology
|March 27, 2024
PubMed
Abstract

Insights

The long non-coding RNA H19 is overexpressed in nodular goiter. Researchers identified NKX2-1 as a target of H19, suggesting a role in goiter development.

Area of Science:

  • Molecular Biology
  • Genetics
  • Endocrinology

Background:

  • Nodular goiter may increase thyroid cancer risk, with unclear genetic factors.
  • H19 long non-coding RNA (lncRNA) is overexpressed in goiter tissue, but its target is unknown.

Purpose of the Study:

  • To identify a novel target for H19 lncRNA in the context of nodular goiter development.
  • Investigate the interaction between H19 lncRNA and NKX2-1 mRNA.

Main Methods:

  • Interaction energy calculations to analyze H19-NKX2-1 binding.
  • RNA sequencing to assess gene expression tissue specificity.
  • RT-qPCR to quantify NKX2-1 expression in 18 goiter and 18 normal thyroid tissues.

Main Results:

  • H19 binds to NKX2-1 mRNA with high affinity, overlapping with has-miR-1827 binding site.
  • NKX2-1 is co-expressed with H19 exclusively in normal thyroid tissue.
  • NKX2-1 is significantly overexpressed in nodular goiter tissues compared to normal tissues (p=0.038).

Conclusions:

  • NKX2-1 is identified as a putative target of H19 lncRNA.
  • H19 lncRNA overexpression in nodular goiter is linked to NKX2-1 dysregulation.
Keywords:
Gene expressionNKX2-1Nodular goiter

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