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Related Experiment Video

Updated: Jun 29, 2025

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Neuronal subtype-specific transcriptomic changes in the cerebral neocortex associated with sleep pressure.

Shinya Nakata1, Kanako Iwasaki1, Hiromasa Funato2

  • 1International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan.

Neuroscience Research
|March 27, 2024
PubMed
Summary
This summary is machine-generated.

Sleep deprivation significantly alters gene expression in specific neocortical neurons. Layer 2/3 intratelencephalic (L2/3 IT) neurons are most affected, with Junb identified as a key transcription factor.

Keywords:
NeocortexSIK3Sleep deprivationSleep pressuresnRNA-seq

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Area of Science:

  • Neuroscience
  • Transcriptomics
  • Sleep Regulation

Background:

  • Sleep pressure is a homeostatic mechanism regulated by sleep and wakefulness.
  • The cerebral neocortex, with its diverse neuronal subtypes, is implicated in sleep pressure regulation via transcriptional control.

Purpose of the Study:

  • To investigate transcriptomic changes in neocortical neuronal subtypes under increased sleep pressure.
  • To identify molecular players, including transcription factors and intercellular ligands, involved in sleep pressure-induced transcriptome alterations.

Main Methods:

  • Analysis of single-nucleus RNA sequencing datasets.
  • Examination of transcriptomic changes in neocortical neurons following sleep deprivation (SD).
  • Assessment of transcriptomic effects of mutant SIK3 (SLP) expression.

Main Results:

  • Sleep deprivation (SD) most profoundly impacted the transcriptome of layer 2 and 3 intratelencephalic (L2/3 IT) neurons among neocortical glutamatergic subtypes.
  • Mutant SIK3 (SLP) expression also induced significant transcriptomic changes in L2/3 IT neurons.
  • Junb was identified as a candidate transcription factor mediating these alterations.
  • Putative intercellular ligands, including BDNF, LSAMP, and PRNP, were inferred to be involved in SD-induced transcriptomic changes in L2/3 IT neurons.

Conclusions:

  • The transcriptome of L2/3 IT neurons is particularly sensitive to increased sleep pressure.
  • Junb plays a crucial role in mediating sleep pressure-induced transcriptional changes in L2/3 IT neurons.
  • Specific intercellular ligands may mediate the communication underlying these transcriptomic alterations in L2/3 IT neurons.