Identification of Down-Expressed CRNN Associated with Cancer Progression and Poor Prognosis in Laryngeal Squamous Cell Carcinoma.
Feilong Hong1, Xuemei Wan2, Yundan Bai3
1Department of Otolaryngology, Hangzhou Hospital of Zhejiang Medical and Health Group, 310022 Hangzhou, Zhejiang, China.
Frontiers in Bioscience (Landmark Edition)
|March 27, 2024
View abstract on PubMed
Summary
Cornulin (CRNN) is downregulated in laryngeal squamous cell carcinoma (LSCC), suppressing tumor growth and progression. CRNN acts as a tumor suppressor, offering potential therapeutic targets for LSCC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Laryngeal squamous cell carcinoma (LSCC) prevalence is rising, necessitating the identification of novel therapeutic targets.
- Understanding the molecular mechanisms underlying LSCC is critical for developing effective treatments.
Purpose of the Study:
- To identify key genes involved in LSCC development and progression.
- To investigate the role of cornulin (CRNN) as a potential therapeutic target in LSCC.
Main Methods:
- Bioinformatic analysis of differentially expressed genes (DEGs) in LSCC.
- Immunohistochemistry and Western blotting for protein expression analysis.
- In vitro assays (MTT, Edu, flow cytometry, wound healing, transwell) and in vivo studies in nude mice.
Main Results:
- Identified 36 upregulated and 65 downregulated DEGs in LSCC, with CRNN as a key hub gene.
- CRNN expression was consistently downregulated in LSCC tissues and cell lines, correlating with poor patient survival.
- CRNN overexpression inhibited LSCC cell growth, cell cycle progression, migration, and invasion, and suppressed tumor growth in vivo.
Conclusions:
- CRNN functions as a tumor suppressor in LSCC.
- CRNN plays a significant role in LSCC pathogenesis and presents a potential target for therapeutic interventions.
More Related Videos
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
01:22MicroRNAs
21.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.3K
01:15Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
03:18Non-LTR Retrotransposons
11.5K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.5K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K