Real-time analysis of sperm motion using automatic video image digitization

A methodology is described for automatic, real-time analysis of sperm movement characteristics. Video micrographic images of spermatozoa are digitized, compressed, and then analyzed using a Motion Analysis Corporation Expert Vision system. The system includes a VP-100 video processor, a video camera, and a Sun-2/120 minicomputer. Algorithms for detection of and discrimination among sperm are described, and desirable operating characteristics of the system are considered. Salient parameters which characterize the kinematics of sperm swimming trajectories are discussed.