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Updated: Jun 29, 2025

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
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Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

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Molecular Targets of Oxidative Stress: Focus on the Nrf2 Signaling Pathway in Health and Disease

Marcel Bonay1,2

  • 1UVSQ, INSERM END-ICAP, Université Paris-Saclay, 78000 Versailles, France.

Antioxidants (Basel, Switzerland)
|March 28, 2024
PubMed

Insights

Oxidative stress, an imbalance in reactive oxygen species (ROS) and antioxidants, elevates risks for metabolic disorders, chronic diseases, and cancer. Understanding this imbalance is crucial for preventing and treating these conditions.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pathophysiology

Background:

  • Oxidative stress arises from an imbalance between reactive oxygen species (ROS) production and antioxidant defenses.
  • This imbalance is implicated in the pathogenesis of numerous chronic and metabolic disorders, including cancer.

Discussion:

  • The detrimental effects of ROS accumulation contribute to cellular damage and dysfunction.
  • Antioxidant capacity plays a critical role in mitigating oxidative damage.

Key Insights:

  • Oxidative stress is a significant risk factor for metabolic diseases and cancer.
  • Maintaining antioxidant balance is essential for cellular health and disease prevention.

Outlook:

  • Further research into antioxidant mechanisms can reveal novel therapeutic targets.
  • Strategies to bolster antioxidant capacity may offer protective benefits against oxidative stress-related diseases.

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