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Transcriptome Responses to Different Salinity Conditions in Litoditis marina, Revealed by Long-Read Sequencing
Pengchi Zhang1,2,3,4, Beining Xue1,2,3,4, Hanwen Yang1,2,3
1CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Genes
|March 28, 2024
Summary
This study used long-read sequencing to analyze marine nematode transcriptome responses to varying salinity levels. It identified novel transcripts and key genes involved in salinity adaptation, revealing insights into euryhalinity mechanisms.
Area of Science:
- Marine biology
- Genomics
- Molecular biology
Background:
- The marine nematode *Litoditis marina* exhibits global distribution in intertidal zones.
- Mechanisms of its broad salinity adaptation (euryhalinity) are not well understood.
Purpose of the Study:
- To investigate the transcriptome responses of *L. marina* to different salinity conditions using Oxford Nanopore (ONT) long-read sequencing.
- To identify genes and pathways involved in salinity tolerance and adaptation.
Main Methods:
- ONT long-read sequencing of *L. marina* under 3‰, 30‰, and 60‰ salinity.
- Analysis of differentially expressed genes (DEGs) and Gene Ontology (GO) enrichment.
- Investigation of alternative polyadenylation (APA) events.
Main Results:
- Generated 26,647 non-redundant long-read transcripts, including 6,464 novel ones.
- ONT sequencing data highly correlated with previous Illumina short-read data.
- Identified shared GO terms related to transport and oxidoreductase activity across salinity conditions.
- Observed significant changes in gene expression and APA events with increasing salinity.
- Enriched GO terms related to cellular component assembly and coenzyme biosynthesis under stress.
Conclusions:
- Provides a comprehensive transcriptomic landscape of *L. marina* under varying salinities.
- Highlights the role of specific genes and pathways in euryhalinity.
- Offers insights into salinity adaptation mechanisms relevant to climate change studies.
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