Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Myosin Structure and Function

Overview of Myosin Structure and Function

Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X) have been well...

Conserved Binding Sites

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...

The Sarcomere

The Sarcomere

A sarcomere is a microscopic segment repeating in a myofibril. The sarcomere fundamentally consists of two main myofilaments: thick filaments called myosin and thin filaments called actin. These filaments interact by sliding past each other in response to stimulus. In addition to myosin and actin, several other proteins, such as tropomyosin, troponin, titin, nebulin, myomesin, α-actinin, and dystrophin, play crucial roles in regulating, structuring, and functioning of the sarcomere.
Each...

Protein Organization

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....

Protein Folding

Protein Folding

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Secretin-interacting plug proteins prevent antibiotic influx during type IV pilus assembly in Pseudomonas aeruginosa.

Nature communications·2026

Same author

Bridging the Structural Gap: A Methodological Review of Cryo-Electron Microscopy for Underrepresented Viruses.

Viruses·2026

Same author

Cryo-EM Structures Reveal the Molecular Basis of Asymmetric Allosteric Activation by MMOB in the Hydroxylase of Soluble Methane Monooxygenase.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Identification of a novel compound that induces oligomer disassembly of the pyruvate dehydrogenase E2 complex.

Biochemical and biophysical research communications·2025

Same author

Optimizing MALDI-TOF Mass Spectrometry for the Identification of <i>Bacillus cereus</i>: The Impact of Sporulation and Cultivation Time.

International journal of molecular sciences·2025

Same author

Rocaglamide Suppresses Allergic Reactions by Regulating IL-4 Receptor Signaling.

Molecules (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Jun 29, 2025

Author Spotlight: Unraveling the Role of Myosin-7a and Usher Proteins in Hearing and Human Disease

Author Spotlight: Unraveling the Role of Myosin-7a and Usher Proteins in Hearing and Human Disease

Published on: August 23, 2024

Sequence Alignment-Based Prediction of Myosin 7A: Structural Implications and Protein Interactions.

Chan Jong Yu¹, Yoon Ho Park¹, Bumhan Ryu²

¹Division of Chemistry & Biochemistry, Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.

International Journal of Molecular Sciences

|March 28, 2024

Summary

This study models the myosin 7A motor domain structure, predicting nucleotide and actin binding sites. Insights into the myosin 7A structure and its interactions with actin were revealed.

Keywords:

actomyosin complex multiple sequence alignment pliant region sequence-based structure prediction

More Related Videos

A Protocol for Computer-Based Protein Structure and Function Prediction

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Related Experiment Videos

Last Updated: Jun 29, 2025

Author Spotlight: Unraveling the Role of Myosin-7a and Usher Proteins in Hearing and Human Disease

Author Spotlight: Unraveling the Role of Myosin-7a and Usher Proteins in Hearing and Human Disease

Published on: August 23, 2024

A Protocol for Computer-Based Protein Structure and Function Prediction

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Area of Science:

Biochemistry
Structural Biology
Molecular Motor Function

Background:

Myosin motor proteins utilize ATP hydrolysis for movement and actin binding.
Diverse myosin isoforms perform various cellular functions.
Lack of resolved structures hinders understanding of myosin mechanochemical cycles and functional diversity.

Purpose of the Study:

To elucidate the structural organization of the myosin 7A motor domain.
To model the tertiary structure of myosin 7A from its primary sequence.
To understand how structural diversity relates to distinct functional properties.

Main Methods:

Tertiary structure modeling of myosin 7A based on primary sequence.
Multiple sequence alignment of myosin isoforms.
Protein-protein interaction modeling to predict actomyosin complex.

Main Results:

Identified highly conserved nucleotide binding sites.
Predicted actin binding sites on myosin 7A.
Defined core interface sites within the actomyosin-7A complex.
Suggested a pliant region between myosin 7A's converter domain and lever arm.

Conclusions:

The study provides novel insights into myosin 7A structure and function.
Predicted structural features offer a framework for future high-resolution studies.
Understanding myosin 7A's structural basis can inform research on its diverse roles.