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Engineering Terpene Production Pathways in Methylobacterium extorquens AM1.

Allison Hurt1, Jacob D Bibik2,3, Norma Cecilia Martinez-Gomez4

  • 1Department on Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.

Microorganisms
|March 28, 2024
PubMed
Summary

Engineered methylotrophic bacteria, Methylobacterium extorquens, produced the valuable terpene patchoulol when supplemented with lanthanides. This demonstrates a sustainable method for producing complex molecules using plant-associated microbes.

Keywords:
casbeneinducible expressionlanthanidemetabolismmethylotrophspatchoulolterpenes

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Area of Science:

  • Microbial Biotechnology
  • Synthetic Biology
  • Metabolic Engineering

Background:

  • Terpenes are valuable plant compounds with diverse industrial applications, but their chemical synthesis is complex and costly.
  • Microbial hosts like methylotrophs offer a sustainable alternative for terpene production using renewable feedstocks.
  • Methylotrophs utilize one-carbon compounds and can be influenced by lanthanides, presenting an opportunity for metabolic manipulation.

Purpose of the Study:

  • To engineer methylotrophic bacteria for enhanced terpene production.
  • To investigate the role of lanthanides in boosting terpene biosynthesis in methylotrophs.
  • To explore the potential of plant-associated methylotrophs for sustainable small molecule production.

Main Methods:

  • Engineered wildtype Methylobacterium extorquens, a carotenoid-deficient mutant (∆Meta1_3665), and E. coli with an exogenous terpene pathway.
  • Cultured strains in the presence and absence of lanthanides.
  • Utilized native inducible and constitutive promoters to regulate terpene production.

Main Results:

  • Engineered M. extorquens with a native inducible promoter produced the sesquiterpene patchoulol when supplemented with lanthanide.
  • A moderate-strength constitutive promoter did not yield patchoulol production.
  • Demonstrated successful colonization of the phyllosphere by engineered strains.

Conclusions:

  • Lanthanide supplementation enhances terpene production in engineered methylotrophs.
  • Native inducible promoters are effective for terpene biosynthesis in M. extorquens.
  • Engineering plant microbiome species offers a promising avenue for sustainable small molecule synthesis.