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Negevirus Piura Suppresses Zika Virus Replication in Mosquito Cells

Valéria L Carvalho1, Dhani Prakoso2, Erika R Schwarz3

  • 1Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Rodovia BR-316, Km 7, s/n, Ananindeua 67030-000, PA, Brazil.

Viruses
|March 28, 2024
PubMed

Insights

Piura virus (PIUV) suppresses Zika virus (ZIKV) replication in Aedes albopictus cells. PIUV significantly reduced ZIKV titers by 10,000-fold within 3 days, while ZIKV did not affect PIUV replication.

Area of Science:

  • Virology
  • Arthropod-borne viruses
  • Viral interactions

Background:

  • Zika virus (ZIKV) is a significant arbovirus with global health implications.
  • Piura virus (PIUV) is an insect-specific virus with poorly understood interactions.
  • Aedes albopictus mosquitoes are key vectors for arboviruses like ZIKV.

Purpose of the Study:

  • To investigate the interaction between Piura virus (PIUV) and Zika virus (ZIKV) during coinfection in Aedes albopictus cells.
  • To determine if PIUV or ZIKV interferes with the replication of the other virus.
  • To quantify the impact of PIUV on ZIKV replication dynamics.

Main Methods:

  • Coinfection experiments were conducted in C6/36 Aedes albopictus cells using PIUV and ZIKV.
  • Two multiplicity of infection (MOI) ratios were tested for co-inoculation.
  • Viral loads were assessed daily for 7 days using TCID50 titration and RT-qPCR.

Main Results:

  • Piura virus (PIUV) significantly suppressed Zika virus (ZIKV) replication, reducing ZIKV titers by approximately 10,000-fold within 3 days post-infection.
  • PIUV viral loads remained unaffected in the presence of ZIKV.
  • ZIKV did not interfere with PIUV replication.

Conclusions:

  • Piura virus (PIUV) actively suppresses Zika virus (ZIKV) replication in Aedes albopictus cells.
  • ZIKV does not inhibit PIUV replication when coinfection occurs.
  • These findings highlight a potential mechanism of viral interference in mosquito vectors.
Keywords:
Piura virusZika viruscoinfection

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