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Genetic Diversity of the Human Adenovirus C Isolated from Hospitalized Children in Russia (2019-2022)
Olga G Kurskaya1, Elena A Prokopyeva1, Nikita A Dubovitskiy1
1Laboratory of Molecular Epidemiology and Biodiversity of Viruses, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk 630060, Russia.
Insights
Human adenovirus (HAdV) was studied in hospitalized children with acute respiratory virus infection (ARVI) in Russia. Genotypes C1, C2, C5, C89, and 108 were identified, providing crucial molecular epidemiological data.
Area of Science:
- Virology
- Epidemiology
- Pediatrics
Background:
- Human adenovirus (HAdV) is a frequent cause of acute respiratory virus infection (ARVI) in children.
- Limited molecular epidemiological and clinical data on HAdV in hospitalized Russian children with ARVI exist.
Purpose of the Study:
- To investigate the epidemiological and molecular characteristics of HAdV in hospitalized children with ARVI in Novosibirsk, Russia.
- To analyze the incidence and genotypes of HAdV and co-infections with other respiratory viruses.
Main Methods:
- A 4-year longitudinal study (2019-2022) involving 3190 hospitalized children (0-17 years old) diagnosed with ARVI.
- Real-time polymerase chain reaction (PCR) was used to detect HAdV and other respiratory viruses.
- Full-genome sequencing was performed on 12 isolated HAdV strains.
Main Results:
- At least one respiratory virus was detected in 74.4% of cases; HAdV was detected in 4% of these.
- HAdV co-infections were observed in 1.3% of cases.
- Circulating HAdV genotypes identified were C1, C2, C5, C89, and 108.
Conclusions:
- This study provides novel molecular epidemiological insights into HAdV infections among hospitalized children with ARVI in Russia.
- The identified genotypes highlight the diversity of circulating HAdV strains and inform public health strategies.
Abstract:
The human adenovirus (HAdV) is a common pathogen in children that can cause acute respiratory virus infection (ARVI). However, the molecular epidemiological and clinical information relating to HAdV among hospitalized children with ARVI is rarely reported in Russia. A 4-year longitudinal (2019-2022) study among hospitalized children (0-17 years old) with ARVI in Novosibirsk, Russia, was conducted to evaluate the epidemiological and molecular characteristics of HAdV. Statistically significant differences in the detection rates of epidemiological and virological data of all positive viral detections of HAdV were analyzed using a two-tailed Chi-square test. The incidence of HAdV and other respiratory viruses such as human influenza A and B viruses, respiratory syncytial virus, coronavirus, parainfluenza virus, metapneumovirus, rhinovirus, bocavirus, and SARS-CoV-2 was investigated among 3190 hospitalized children using real-time polymerase chain reaction. At least one of these respiratory viruses was detected in 74.4% of hospitalized cases, among which HAdV accounted for 4%. A total of 1.3% co-infections with HAdV were also registered. We obtained full-genome sequences of 12 HAdVs, which were isolated in cell cultures. Genetic analysis revealed the circulation of adenovirus of genotypes C1, C2, C5, C89, and 108 among hospitalized children in the period from 2019-2022.
