Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells
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View abstract on PubMed
Summary
This summary is machine-generated.The BacMam platform using baculovirus offers superior recombinant protein production in mammalian cells compared to standard transfection methods. This baculovirus system demonstrates efficient transient and stable expression, with enhanced yields and scalability.
Area Of Science
- Biotechnology
- Molecular Biology
- Cell Biology
Background
- Mammalian cell protein production relies on transient transfection or stable cell line generation, both with limitations.
- BacMam, a baculovirus-based transduction system, presents an alternative for recombinant protein expression.
Purpose Of The Study
- To compare BacMam transduction with six transfection agents for transient and stable protein expression.
- To optimize BacMam processes, including expression enhancement and baculovirus purification.
- To evaluate BacMam efficiency across HEK293-6E, CHO-K1, and Vero cell lines.
Main Methods
- Comparative analysis of six transfection agents versus baculovirus transduction.
- Assessment of transient and stable protein expression (ACE2-eGFP model).
- Process optimization including sodium butyrate, TSA, and baculovirus ultracentrifugation.
Main Results
- BacMam transduction significantly outperformed all transfection agents in efficiency across all tested cell lines.
- An 18-fold increase in protein expression was achieved using sodium butyrate enhancement.
- Ultracentrifugation improved baculovirus transduction efficiency and protein yield, demonstrating scale-up feasibility.
Conclusions
- The BacMam platform is superior to standard transfection for mammalian recombinant protein production.
- Baculovirus efficiently transduces diverse cell lines for both transient and stable expression.
- BacMam offers a scalable and efficient solution for recombinant protein manufacturing.
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