Analysis of Fluid Replacement in Two Fluidic Chambers for Oblique-Incidence Reflectivity Difference (OI-RD) Biosensor

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Optical Sensing

Background:

• Optical biosensors are vital tools in diagnostics and research.
• Fluidic chambers are essential for controlled reagent delivery in biosensors.
• Inconsistent fluidic chamber designs hinder reliable liquid replacement and data accuracy.

Purpose of the Study:

• To determine the optimal fluid volume for complete liquid replacement in optical biosensor fluidic chambers.
• To investigate the influence of fluidic chamber geometry and flow rate on liquid replacement efficiency.
• To establish guidelines for fluidic chamber design in oblique-incidence reflectivity difference (OI-RD) biosensors.

Main Methods:

• Utilized COMSOL Multiphysics finite element analysis software for simulation.
• Investigated two distinct fluidic chamber configurations.
• Analyzed the impact of chamber depth, sample positioning, and flow rate on fluid dynamics.

Main Results:

• Fluidic chamber depth was identified as the primary determinant of optimal fluid volume.
• Required fluid volume demonstrated a quadratic relationship with chamber depth.
• Sample surface positions influenced necessary fluid volume, while flow rate had no significant effect.

Conclusions:

• Chamber depth is the most critical parameter for optimizing fluid volume in OI-RD biosensor fluidic chambers.
• Standardized guidelines for fluidic chamber design are needed to ensure reliable liquid replacement.
• This research provides insights for designing more efficient and accurate optical biosensor systems.