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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
In situ imaging of mRNA transcripts based on split-aptamer and split protein in living cells
Yan Peng1,2, Mengqi Li1, Fuyu Gong1,2
1The Affiliated First Hospital of Fuyang Normal University, Fuyang Normal University Fuyang Anhui 236037 P.R. China pengyan@fynu.edu.cn gong2022@fynu.edu.cn.
Abstract:
Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, "turn-on" probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated selectivity, stability, and capability for targeting various mRNAs.
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