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Published on: October 16, 2013
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Isoflurane preconditioning induced genomic changes in mouse cortex
Umeshkumar Athiraman1,2, Tusar Giri1
1Department of Anesthesiology, Washington University, St. Louis, MO, USA.
BJA Open
|March 28, 2024
Summary
Isoflurane preconditioning alters gene expression in the mouse brain cortex, showing a biphasic response over 72 hours. This study reveals temporal changes in genes related to neurodegeneration and nervous system disorders.
Area of Science:
- Neuroscience
- Genomics
- Anesthesiology
Background:
- Altered gene expression patterns in the mouse brain following isoflurane preconditioning remain uninvestigated.
- This pilot study aims to explore the temporal sequence of transcriptome changes in the mouse brain cortex induced by isoflurane preconditioning.
Purpose of the Study:
- To examine the temporal sequence of changes in the transcriptome of mouse brain cortex produced by isoflurane preconditioning.
- To identify molecular mechanisms underlying isoflurane preconditioning-induced neuroprotection.
Main Methods:
- Mice were exposed to isoflurane (2% in air for 1 hour) and brains harvested at multiple time points (0-72 hours).
- A separate cohort received three isoflurane doses over three days.
- Gene expression was analyzed using the NanoString mouse neuropathology panel (760 genes).
Main Results:
- Isoflurane preconditioning upregulated and downregulated genes across several pathways.
- A biphasic gene expression response was observed: an early wave (until 6 hours), a pause (until 24 hours), and a second wave (starting at 36 hours).
Conclusions:
- Isoflurane preconditioning significantly alters genes involved in neurodegeneration and nervous system disorders over time.
- These findings may help elucidate molecular mechanisms of isoflurane-induced neuroprotection in central nervous system diseases.
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