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In Vivo Fluorescent Labeling of Foam Cell-Derived Extracellular Vesicles as Circulating Biomarkers for In Vitro Detection of Atherosclerosis

  • 0State Key Laboratory of Medicinal Chemical Biology, Frontiers Science Centers for Cell Responses and New Organic Matter, Research Center for Analytical Sciences, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, College of Chemistry, Nankai University, Tianjin 300071, China.
Journal of the American Chemical Society +

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March 28, 2024

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March 28, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Researchers developed a novel in vivo fluorescent nanoprobe for early atherosclerosis (AS) detection. This method specifically labels foam cell-derived extracellular vesicles (EVs) in blood, enabling sensitive, real-time monitoring of AS progression and cardiovascular disease (CVD) management.

Area Of Science

  • Biomedical Engineering
  • Cardiovascular Research
  • Nanotechnology

Background

  • Atherosclerosis (AS) development monitoring is crucial for cardiovascular disease (CVD) management.
  • Current laboratory methods for AS detection lack sensitivity and specificity due to limited reliable biomarkers.
  • Foam cell-derived extracellular vesicles (EVs) are potential circulating biomarkers for AS.

Purpose Of The Study

  • To develop a sensitive and specific in vivo fluorescent labeling strategy for detecting AS.
  • To enable real-time monitoring of AS progression and lesion vulnerability.
  • To establish a liquid-biopsy approach for early AS detection and CVD management.

Main Methods

  • Developed a self-assembled nanoprobe for specific recognition of foam cells in atherosclerotic plaques.
  • The nanoprobe is degraded intracellularly by HClO, releasing a lipophilic fluorophore (B-CF3) that stains EVs.
  • Circulating B-CF3-stained EVs are detected using fluorescence spectrometry or microplate readers.

Main Results

  • The strategy allows specific in vivo fluorescent staining of foam cell-derived EVs in atherosclerotic plaques.
  • B-CF3-stained EVs are released into the blood, serving as detectable circulating biomarkers for AS.
  • The method enables direct detection of EVs without complex analytical techniques, facilitating early AS diagnosis.

Conclusions

  • The developed fluorescent labeling strategy provides a sensitive and specific method for AS detection.
  • This liquid-biopsy approach allows for early detection and real-time differentiation of lesion vulnerability in AS progression.
  • The findings facilitate effective cardiovascular disease management through improved AS monitoring.