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Related Experiment Video

Updated: Jun 12, 2026

Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
10:16

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Towards a reference material for microplastics' number concentration-case study of PET in water using Raman

Oliver Jacob1, Elżbieta Anna Stefaniak2, John Seghers2

  • 1Institute of Water Chemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Lichtenbergstr. 4, 85748, Garching, Germany.

Analytical and Bioanalytical Chemistry
|March 28, 2024
PubMed
Summary

A new reference material (RM) for polyethylene terephthalate (PET) microplastics in water was developed. This RM demonstrates sufficient homogeneity and stability for validating microplastic quantification methods.

Keywords:
HomogeneityMicroplasticsParticle number concentrationRaman microspectroscopyReference materialStability

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Materials Science

Background:

  • Accurate identification and quantification of microplastics in water samples are crucial.
  • Method validation for microplastic analysis relies on certified reference materials (CRMs).

Purpose of the Study:

  • To assess the suitability of a newly developed polyethylene terephthalate (PET) microplastic in water candidate reference material (RM).
  • To determine the homogeneity and stability of microplastic particle number concentration within the candidate RM.

Main Methods:

  • Utilized Raman microspectroscopy for particle identification and quantification.
  • Employed automated particle counting and analysis software (TUM-ParticleTyper 2).
  • Evaluated particle number concentration and size distribution across multiple RM units.

Main Results:

  • The candidate RM exhibited homogeneity for PET microplastic particle counts (>30 µm) with a relative standard deviation of 12%.
  • Total particle counts and counts within specific size classes were comparable across all tested RM units.
  • Demonstrated stability of particle number concentration in the microplastic RM.

Conclusions:

  • The developed PET microplastic in water RM is sufficiently homogeneous and stable.
  • This RM is suitable for validating methods used in microplastic analysis.
  • Facilitates reliable size-resolved identification and quantification of microplastics in environmental and drinking water samples.