Related Experiment Video
Updated: Jun 29, 2025
08:47
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
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eIF4F is a thermo-sensing regulatory node in the translational heat shock response
Christine Desroches Altamirano1, Moo-Koo Kang1, Mareike A Jordan2
1Biotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering, Technische Universität Dresden, Tatzberg 47/49, 01307 Dresden, Germany.
Molecular Cell
|March 28, 2024
Summary
Heat shock (HS) causes cells to prioritize HS mRNA translation. The eIF4F complex acts as a thermo-sensor, dissociating to form granules that repress housekeeping translation while allowing HS mRNA translation.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Heat shock (HS) induces selective mRNA translation.
- The mechanism of HS-induced translation regulation is not fully understood.
Purpose of the Study:
- To elucidate the mechanism by which heat-shocked cells prioritize heat shock mRNA translation.
- To identify the role of the eIF4F complex in heat shock response.
Main Methods:
- In vitro reconstitution biochemistry.
- Budding yeast cell extracts and cellular experiments.
Main Results:
- Heat shock (HS) causes disassembly of the eIF4F complex.
- eIF4G undergoes conformational changes, dissociating eIF4A.
- Formation of heat shock messenger ribonucleoprotein particles (mRNPs) and condensates represses housekeeping translation.
- Heat shock mRNAs are preferentially translated by eIF4A.
Conclusions:
- The eIF4F complex functions as a thermo-sensing node.
- Regulation of translation during heat shock is mediated by eIF4F complex dynamics.
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