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Label-Free Visualization and Morphological Profiling of Neuronal Differentiation and Axonal Degeneration through

Jeong Hee Kim1, Aysel Cetinkaya-Fisgin2, Noah Zahn3

  • 1Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.

Advanced Biology
|March 28, 2024
PubMed
Summary

Quantitative phase imaging (QPI) offers a label-free method to monitor neuronal growth and degeneration, aiding in understanding neurotoxicity and improving treatments for conditions like chemotherapy-induced peripheral neuropathy (CIPN). This non-invasive technique quantifies cellular changes, advancing neurotoxicity management.

Keywords:
axon degenerationchemotherapy‐induced peripheral neuropathy (CIPN)dorsal root ganglion (DRG)label‐free imagingquantitative phase imaging (QPI)

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biomedical Imaging

Background:

  • Neuronal growth, degeneration, and neurotoxicity are crucial for nervous system function and patient outcomes, particularly in chemotherapy-induced peripheral neuropathy (CIPN).
  • Conventional methods for assessing neuronal morphological changes, such as neurite outgrowth and axon length, often require extensive sample preparation and manual measurements.
  • Phenotypic cellular changes are intrinsically linked to the progression of neuronal processes.

Purpose of the Study:

  • To introduce and validate a label-free, non-invasive quantitative phase imaging (QPI) approach for monitoring neuronal differentiation and degeneration.
  • To demonstrate QPI's capability in visualizing and quantifying physical properties of neurons, including axon length.
  • To explore QPI's utility in understanding neuronal responses to stimuli simulating CIPN conditions.

Main Methods:

  • Utilized quantitative phase imaging (QPI), a label-free and non-invasive microscopy technique.
  • Applied QPI to unlabeled dorsal root ganglion (DRG) neuronal cells.
  • Quantified cellular morphology and dynamics through optical path length delay maps.
  • Simulated conditions mimicking chemotherapy-induced peripheral neuropathy (CIPN).

Main Results:

  • QPI successfully provided label-free, non-invasive monitoring of neuronal differentiation and degeneration.
  • The technique generated quantitative maps of optical path length delays, enabling objective measurement of cellular morphology and dynamics.
  • QPI allowed for the visualization and quantification of axon length and other physical properties of DRG neurons.
  • Neuronal responses to simulated CIPN conditions were effectively studied using QPI.

Conclusions:

  • Quantitative phase imaging (QPI) presents a powerful, non-invasive tool for studying neuronal processes like growth and degeneration.
  • This method overcomes limitations of conventional techniques, offering objective and detailed insights into cellular morphology and dynamics.
  • QPI facilitates a deeper understanding of neurotoxicity, paving the way for improved clinical management strategies for conditions such as CIPN.